Mol. Hum. Reprod. Advance Access originally published online on March 3, 2006
Molecular Human Reproduction 2006 12(4):233-236; doi:10.1093/molehr/gal024
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Genetic polymorphisms of vascular endothelial growth factor in severe pre-eclampsia
1First Department of Pediatrics, 2Szentágothai János Knowledge Centre, Semmelweis University, 3Research Group for Pediatrics and Nephrology, Hungarian Academy of Sciences and 4First Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary
5 To whom correspondence should be addressed at: First Department of Pediatrics, Semmelweis University, 1083 Budapest, Bókay János u. 53-54, Hungary. E-mail: banyasz{at}gyer1.sote.hu
 |
Abstract |
|---|
 Top Abstract IntroductionResultsDiscussionReferences |
|---|
Several lines of evidence support the hypothesis that vascular endothelial growth factor (VEGF) plays an important role in the pathogenesis of pre-eclampsia (PE). VEGF is a key component in the regulation of vascular remodelling and the survival of cytotrophoblasts in the placenta. In this case–control study, we aimed to test whether VEGF genetic polymorphisms are associated with the risk of severe PE. We enrolled 84 nulliparous pregnant women with severe PE (PE group). Their VEGF G+405C and VEGF C–2578A genotypes were determined by PCR-restriction fragment length polymorphism (PCR-RFLP) from venous blood samples and were compared with the corresponding VEGF genotypes of 96 nulliparous patients with uncomplicated pregnancies (control group). Carriers of the VEGF+405G allele occurred less frequently in PE than in the control group [P = 0.039; adjusted odds ratio (aOR) = 0.28, range: 0.08–0.93]. Hypertension and proteinuria were diagnosed earlier (by 1.6 weeks and 1.9 weeks, respectively) in PE patients with VEGF–2578A only after adjustment of this association for risk factors of PE. Our results suggest that carriers of VEGF+405G allele have a decreased susceptibility to PE and that the progression of PE may be modified by the presence of VEGF–2578A allele. Nevertheless, the clinical significance of these findings remains to be determined.
Key words: hypertension/polymorphism/proteinuria/severe pre-eclampsia/VEGF
|
Introduction |
|---|
|
TopAbstractIntroductionResultsDiscussionReferences |
|---|
Pre-eclampsia (PE) is a multi-organ disorder defined as elevated blood pressure (>140/90 mmHg) and proteinuria that has an onset after the 20th week of gestation. PE affects 5 to 8% of pregnant women depending on the population studied and definitions of PE (ACOG Committee on Practice Bulletins—Obstetrics, 2002; Redman and Sargent, 2005
).
Several mechanisms have been suggested to play a role in PE. One of the theories emphasizes the possible role of improper placentation. Placentation is a complex process that requires the invasion of cytotrophoblast into the decidual and intramyometrial portions of the spiral arteries of the uterus. As a result, the spiral arteries lose their elasticity, and their diameter increases. Blood flow is increased to satisfy the intensifying demands of the growing fetus (Kaufman et al., 2003). In PE, vascular remodelling is disturbed, probably because of insufficient cytotrophoblast invasion. Therefore, vascular dilatation does not occur in spiral arteries and utero-placental blood flow decreases, leading to local placental hypoxia (Wang and Alexander, 2000).
Vascular endothelial growth factor (VEGF) is a hypoxia-induced growth factor that is produced by cytotrophoblasts. In cultured cytotrophoblasts, increased mRNA and protein levels of VEGF have been observed in response to hypoxia (Li et al., 2005). Zhou et al. found that cytotrophoblast differentiation and invasion during pregnancy is regulated through VEGF receptor-2. They also found that VEGF regulates cytotrophoblast survival and that expression is dysregulated in severe forms of PE (Zhou et al., 2002). VEGF is a component in the regulation of placentation through its effects on vascular remodelling and the survival and invasion of cytotrophoblast.
Several studies have been performed to elucidate its significance in PE. Some authors observed high levels of VEGF in PE that correlate with the severity of the disease (Ong et al., 2000). Several groups have demonstrated that circulating free VEGF concentrations are significantly lower in women with PE (Lyall et al., 1997; Maynard et al., 2003). Levine et al. demonstrated that serum concentration of soluble fms-like tyrosine kinase 1 (sFlt-1), a natural antagonist of VEGF, is increased in PE, and in parallel there is a decrease in the serum level of free VEGF (Levine et al., 2004). These data suggest the potential role of VEGF in the pathogenesis of the disease. In this study, we hypothesized that a maternal genetic component may contribute to altered VEGF production.
The VEGF gene is highly polymorphic. More than 80 single-nucleotide polymorphisms (SNPs) are known in this region of the human genome (NCBI, Gene accession no.: NT 007592). Some of these such as VEGF G+405C and VEGF C–2578A SNPs may have an impact on VEGF production in peripheral blood mononuclear cells (PBMCs) (Watson et al., 2000; Shahbazi et al., 2002) and, presumably, in other tissues. We tested whether these SNPs are associated with the risk and characteristics of severe PE.
Patients and methods
Patients
In this case–control study, we consecutively enrolled 84 nulliparous pregnant women with severe PE at the First Department of Obstetrics and Gynaecology at Semmelweis University Budapest, Hungary between 1998 and 2004. All participants were of Hungarian ethnicity (Caucasian race). The diagnosis of PE was determined by the American College of Obstetricians and Gynecologists criteria (ACOG Committee on Practice Bulletins—Obstetrics, 2002). Criteria for severe PE were as follows: blood pressure >160/110 mmHg persisting for at least 6 h; and proteinuria >5 g/day after the 20th week of gestation. Blood pressure was measured with a mercury sphygmomanometer and the Korotkov sound technique. Diastolic pressure was indicated by the Korotkov V. sound. All patients had proteinuria 
3+ or 4+ tested by dipstick in at least two random urine specimens obtained at least 4 h apart or protein excretion rate exceeded 5000 mg/24 h. Additionally, at least one of the following symptoms was present: headache, visual disturbance, epigastric pain, dyspnoea, oliguria (urine 
400 ml/24 h), thrombocytopenia (100 000/µl), elevated concentration of lactic dehydrogenase (LDH) (>600 U/l) and aspartate aminotransferase and alanine aminotransferase activity (AST and ALT 70 U/l). There were 12 patients with HELLP syndrome (defined as the common presence of haemolysis, elevated liver enzymes and thrombocytopenia). Patients with chronic hypertension, pre-gestational diabetes and chronic renal disease were excluded. Randomly selected 96 nulliparous women with uncomplicated pregnancies served as controls. Medical history, maternal age, gestational age at delivery and smoking habits were recorded. Pre-pregnancy BMI was calculated as weight in kilograms divided by height in metres squared (for clinical characteristics, see Table I).
|
The enrolled patients were informed in detail about the study and provided their written consent to collect venous blood samples for diagnostic and scientific purposes including genotyping. The study was approved by the Scientific Ethical Committee of the Semmelweis University (No: 148/ 1998) in accordance with the declaration of Helsinki.
Genotyping
DNA was extracted with standardized DNA-extracting protocols using Proteinase K enzyme (Roche Diagnostics, Mannheim, Germany). The amount of used blood samples for the DNA extraction was 500 µl. The PCRs were performed in a final volume of 50 µl containing 10% 10x PCR buffer, 2 mM MgCl2, 0.2 mM dNTPs, 1.5 U Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA) and sense and antisense primers, 0.5 µM of each. We used 100 ng DNA per PCR reaction. The investigated DNA sequences were amplified by the following primers: VEGF C–2578A: forward: 5'-GGGCCTTAGGACACCATACC-3' and reverse: 5'-TGCCCCAGGGAACAAAGT-3'; VEGF G+405C SNP: forward: 5'-CCGACGGCTTGGGGAGATTG-3 and reverse: 5'-CGGCGGTCACCCCCAAAAG-3'.
The condition of PCRs was as follows: 20 s at 94°C (denaturing), 20 s at 57°C (VEGF C–2578A) or at 60°C (VEGF G+405C) (annealing) and 30 s at 72°C (extension) for 40 cycles.
For the VEGF C–2578A, the resultant 267 bp length product was subsequently digested by Bgl II restriction endonuclease (Fermentase Inc., Hanover, MD, USA) at 37°C overnight. This resulted in two fragments 208 and 60 bp in the presence of VEGF–2578A allele. For the VEGF G+405C, the resultant 197 bp length product was subsequently digested by BsmF I restriction endonuclease (New England Biolabs, Beverly, MA, USA) at 65°C overnight. This resulted in two fragments, 167 and 30 bp, in the presence of VEGF+405G allele. The PCR products and digested PCR products were then run on 3% agarose gel followed by ethidium bromide staining. Determination of VEGF G+405C genotype was not successful in three cases.
Data analysis
Hardy–Weinberg equilibrium of the tested SNPs were calculated using the Arlequin software (http://lgb.unige.ch/arlequin/). Logistic regression analysis was used for the analysis of the association between VEGF genotype and risk of severe PE. Multiple linear regression analysis was used to test the association between carrier state of VEGF genotypes and haplotypes and diagnosis date of hypertension and proteinuria. The associations were adjusted for maternal age, BMI before pregnancy and smoking during pregnancy (Lain et al., 1999; Duckitt and Harrington, 2005). All calculations were performed with the statistical software package SPSS 10.0.
|
Results |
|---|
|
TopAbstractIntroductionResultsDiscussionReferences |
|---|
The genotype distribution of the investigated VEGF SNPs fulfilled Hardy–Weinberg criteria in the PE and control groups. Table I summarizes the genotype distribution of VEGF G+405C and VEGF C–2578A polymorphisms.
The results of logistic regression analysis indicated that heterozygous and homozygous carrier states of VEGF+405G alleles presented an independent protective factor in severe PE [P = 0.039, OR (range): 0.28 (0.08–0.93)]. This association was adjusted for the risk factors of PE (maternal age, BMI before pregnancy and smoking during pregnancy) (Lain et al., 1999; Duckitt and Harrington, 2005) (for details, see Table II).
|
Multiple linear regression analysis revealed that in carriers of VEGF–2578A allele, hypertension (P = 0.014, B = –1.64, ß = –0.27) and proteinuria (P = 0.03, B = –1.89, ß = –0.34) were diagnosed earlier (by 1.6 and 1.9 weeks, respectively) (Table III). Hypertension and proteinuria were diagnosed later in carriers of VEGF+405GC/–2578CC haplotype by 2.8 (P = 0.001, B = 2.77, ß = 0.37) and 1.8 weeks (P = 0.027, B = 1.78, ß = 0.25), respectively (data not shown). The associations were adjusted for risk factors enlisted above. (‘B’ refers to the raw, whereas ‘ß’ refers to the standardized regression coefficient.)
|
|
Discussion |
|---|
|
TopAbstractIntroductionResultsDiscussionReferences |
|---|
Our results suggest that the presence of the VEGF+405G allele in nulliparous pregnant women is associated with a decreased risk of severe PE. The association is independent of examined risk factors of PE (maternal age, BMI before pregnancy and smoking during pregnancy). Furthermore, among nulliparous pregnant women with severe PE, carrier state of the VEGF–2578A allele may be associated with the accelerated development of disease.
Until now, only one study has dealt with the association of VEGF SNPs and the risk of PE. Papazoglou et al. demonstrate an association between the most severe form of PE and the VEGF C+936T SNP. They also investigated VEGF G+405C and VEGF C–2578A polymorphisms; however, there was no significant association between these SNPs and PE (Papazoglou et al., 2004). Probably, the explanation for the different results is the different study design. Papazoglou et al. enrolled post-menopausal women with uncomplicated pregnancies in their medical history, whereas we used nulliparous women with healthy pregnancy as controls. As age may be a confounding factor in genetic epidemiological studies (Arason et al., 2003), to avoid this bias, we selected an age-matched control group.
Recently, it has been revealed that the VEGF G+405C and VEGF C–2578A polymorphisms determine the production of VEGF. Significant correlation was observed between the VEGF G+405C and VEGF C–2578A SNPs and the VEGF production of lipopolysaccharide-induced PBMCs. Highest VEGF production was associated with the VEGF+405GG genotype; intermediate production was associated with the GC genotype and lowest production with the CC genotype (Watson et al., 2000). In the case of the VEGF C–2578A SNP, the CC homozygous PMBCs produced significantly more VEGF than AA homozygous cells (Shahbazi et al., 2002).
Our results suggest that genetic polymorphisms of VEGF gene, linked to an inherited alteration of VEGF production, may contribute to the pathogenesis of PE. We found that carrier state of the VEGF+405G allele, which is accompanied by high VEGF-producing capability, decreases the risk of severe PE. VEGF has a central role in many processes that are involved in the development and progression of PE. VEGF is known to play a role in the regulation of cytotrophoblast invasion and placentation (Zhou et al., 2002). It could be hypothesized that our observation that an inherited increase of VEGF-producing ability could be protective against PE is in relation to the earlier experience that VEGF has a potential effect on placentation.
As hypertension and proteinuria were diagnosed earlier in the presence of the VEGF–2578A allele, we also speculate that the progression of PE may be accelerated in the presence of polymorphisms which predispose to low VEGF-producing capacity.
Several lines of evidence support that VEGF has an impact on the regulation of systemic blood pressure. VEGF is known to induce endothelium-dependent vasodilatation in vivo (Horowitz et al., 1997). Systemic administration of VEGF causes hypotension in a dose-dependent manner (Yang et al., 1996). The VEGF-induced hypotension is probably mediated by nitric oxide (Lopez et al., 1997) and prostacyclin (He et al., 1999). In line with these data, we found association between the earlier onset of hypertension and the carrier state of the VEGF–2578A allele, which decreases the VEGF-producing capability (Shahbazi et al., 2002).
VEGF also has an important role in maintaining the integrity of the glomerular filtration barrier, as inhibition of VEGF activity may lead to proteinuria (Eremina et al., 2003; Bdolah et al., 2004). This effect of VEGF in the regulation of glomerular filtration may account for the association between the earlier onset of proteinuria and the carrier state of the VEGF–2578A allele, which predisposes to low production of VEGF (Shahbazi et al., 2002).
Even though the hypertension and proteinuria were diagnosed earlier in the presence of the VEGF–2578A allele after adjustment of risk factors, the clinical significance of this result could be queried, and further investigation is needed to establish it.
It is tempting to speculate that differing VEGF production is the reason for these associations. Our case–control analysis is not suitable for testing this theory. The causative role of these genetic variants in severe PE should be tested by determining VEGF levels in different genotypes of VEGF G+405C and VEGF C–2578A both in the healthy reference population and in women with severe PE.
It is also conceivable that the observed association is not directly related to VEGF production capacity. The VEGF gene is located near to the major histocompatibility complex (MHC) region on chromosome 6. The genes of several other proteins that are possibly implicated in PE [e.g. heat shock protein 70 (Jirecek et al., 2002), tumour necrosis factor (TNF)-
(Conrad and Benyo, 1997)] are also located here. Therefore, it is possible that the observed association between severe PE and VEGF genotypes is the result of linkage disequilibrium with other neighbouring gene polymorphisms.
In conclusion, we found that the presence of the VEGF+405G allele in nulliparous pregnant women is associated with a decreased risk of severe PE. Furthermore, our results suggest that in addition to the risk, the progression of severe PE may also be modified by VEGF polymorphisms; however, to confirm this hypothesis, further prospective studies are needed.
|
Acknowledgements |
|---|
|
TopAbstractIntroductionResultsDiscussionReferences |
|---|
This study was supported by grants OTKA T046086, NKFP 1A/002/2004 and GVOP 3.1.1/2004-300. We are also grateful for the help provided by Ágota Vér, Andrea Fekete and Klára Rosta.
|
References |
|---|
|
TopAbstractIntroductionResultsDiscussionReferences |
|---|
ACOG Committee on Practice Bulletins—Obstetrics (2002) ACOG practice bulletin. Diagnosis and management of preeclampsia and eclampsia. Number 33, January 2002. Obstet Gynecol 99,159–167.[CrossRef][Web of Science][Medline]
Arason GJ, Bodvarsson S, Sigurdarson ST, Sigurdsson G, Thorgeirsson G, Gudmundsson S, Kramer J and Fust G (2003) An age-associated decrease in the frequency of C4B*Q0 indicates that null alleles of complement may affect health or survival. Ann N Y Acad Sci 1010,496–499.[CrossRef][Web of Science][Medline]
Bdolah Y, Sukhatme VP and Karumanchi SA (2004) Angiogenic imbalance in the pathophysiology of preeclampsia: newer insights. Semin Nephrol 24,548–556.[Web of Science][Medline]
Conrad KP and Benyo DF (1997) Placental cytokines and the pathogenesis of preeclampsia. Am J Reprod Immunol 37,240–249.
Duckitt K and Harrington D (2005) Risk factors for pre-eclampsia at antenatal booking: systematic review of controlled studies. BMJ 330,565–567.
Eremina V, Sood M, Haigh J, Nagy A, Lajoie G, Ferrara N, Gerber HP, Kikkawa Y, Miner JH and Quaggin SE (2003) Glomerular-specific alterations of VEGF-A expression lead to distinct congenital and acquired renal diseases. J Clin Invest 111,707–716.[CrossRef][Web of Science][Medline]
He H, Venema VJ, Gu X, Venema RC, Marrero MB and Caldwell RB (1999) Vascular endothelial growth factor signals endothelial cell production of nitric oxide and prostacyclin through flk-1/KDR activation of c-Src. J Biol Chem 274,25130–25135.
Horowitz JR, Rivard A, van der Zee R, Hariawala M, Sheriff DD, Esakof DD, Chaudhry GM, Symes JF and Isner J (1997) Vascular endothelial growth factor produces nitric oxide-dependent hypotension: evidence for a maintenance role in quiescent adult endothelium. Arterioscler Thromb Vasc Biol 17,2793–2800.
Jirecek S, Hohlagschwandtner M, Tempfer C, Knofler M, Husslein P and Zeisler H (2002) Serum levels of heat shock protein 70 in patients with preeclampsia: a pilot-study. Wien Klin Wochenschr 114,730–732.[Web of Science][Medline]
Kaufman P, Black S and Huppertz B (2003) Endovascular trophoblast invasion: implications for pathogenesis intrauterine growth retardation preeclampsia. Biol Reprod 69,1–7.
Lain KY, Powers RW, Krohn MA, Ness RB, Crombleholme WR and Roberts JM (1999) Urinary cotinine concentration confirms the reduced risk of preeclampsia with tobacco exposure. Am J Obstet Gynecol 181,1192–1196.[CrossRef][Web of Science][Medline]
Levine RJ, Maynard SE, Qian C, Lim KH, England LJ, Yu KF, Schisterman EF, Thadhani R, Sachs BP, Epstein FH et al. (2004) Circulating angiogenic factors and the risk of preeclampsia. N Engl J Med 350,672–683.
Li H, Gu B, Zhang Y, Lewis DF and Wang Y (2005) Hypoxia-induced increase in soluble Flt-1 production correlates with enhanced oxidative stress in trophoblast cells from the human placenta. Placenta 26,210–217.[CrossRef][Web of Science][Medline]
Lopez JJ, Laham RJ, Carrozza JP, Tofikuji M, Sellkle F, Bunting S and Simons M (1997) Hemodynamic effects of intracoronary VEGF delivery: evidence of tachyphylaxis and NO dependence of response. Am J Physiol 273,H1317–H1323.
Lyall F, Greer IA, Boswell F and Fleming R (1997) Suppression of serum vascular endothelial growth factor immunoreactivity in normal pregnancy and in pre-eclampsia. Br J Obstet Gynaecol 104,223–228.[Web of Science][Medline]
Maynard SE, Min JY, Merchan J, Lim KH, Li J, Mondal S, Libermann TA, Morgan JP, Sellke FW, Stillman IE et al. (2003) Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia. J Clin Invest 111,649–658.[CrossRef][Web of Science][Medline]
Ong S, Lash G and Baker PN (2000) Angiogenesis and placental growth in normal and compromised pregnancies. Baillieres Best Pract Res Clin Obstet Gynaecol 14,969–980.[CrossRef][Medline]
Papazoglou D, Galazios G, Koukourakis MI, Panagopoulos I, Kontomanolis EN, Papatheodorou K and Maltezos E (2004) Vascular endothelial growth factor gene polymorphisms and pre-eclampsia. Mol Hum Reprod 10,321–324.
Redman CW and Sargent IL (2005) Latest advances in understanding preeclampsia. Science 308,1592–1594.
Shahbazi M, Fryer AA, Pravica V, Brogan IJ, Ramsay HM, Hutchinson IV and Harden PN (2002) Vascular endothelial growth factor gene polymorphisms are associated with acute renal allograft rejection. J Am Soc Nephrol 13,260–264.
Wang Y and Alexander JS (2000) Placental pathophysiology in preeclampsia. Pathophysiology 6,261–270.[CrossRef]
Watson CJ, Webb NJ, Bottomley MJ and Brenchley PE (2000) Identification of polymorphisms within the vascular endothelial growth factor (VEGF) gene: correlation with variation in VEGF protein production. Cytokine 12,1232–1235.[CrossRef][Web of Science][Medline]
Yang R, Thomas G, Bunting S, Ko A, Ferrara N, Keyt B, Ross J and Jin H (1996) Effects of vascular endothelial growth factor on hemodynamic and cardiac performance. J Cardiovasc Pharmacol 27,838–844.[CrossRef][Web of Science][Medline]
Zhou Y, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C and Fisher JS (2002) Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome. Am J Pathol 160,1405–1423.
Submitted on December 9, 2005; accepted on February 2, 2006.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Sharma, A. J. Murphy, M. E. Soto-Quiros, L. Avila, B. J. Klanderman, J. S. Sylvia, J. C. Celedon, B. A. Raby, and S. T. Weiss Association of VEGF polymorphisms with childhood asthma, lung function and airway responsiveness Eur. Respir. J., June 1, 2009; 33(6): 1287 - 1294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. C. Sandrim, A. C. T. Palei, R. C. Cavalli, F. M. Araujo, E. S. Ramos, G. Duarte, and J. E. Tanus-Santos Vascular endothelial growth factor genotypes and haplotypes are associated with pre-eclampsia but not with gestational hypertension Mol. Hum. Reprod., February 1, 2009; 15(2): 115 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. P. Schneider, M. Wang, M. Radovich, G. W. Sledge, S. Badve, A. Thor, D. A. Flockhart, B. Hancock, N. Davidson, J. Gralow, et al. Association of Vascular Endothelial Growth Factor and Vascular Endothelial Growth Factor Receptor-2 Genetic Polymorphisms With Outcome in a Trial of Paclitaxel Compared With Paclitaxel Plus Bevacizumab in Advanced Breast Cancer: ECOG 2100 J. Clin. Oncol., October 1, 2008; 26(28): 4672 - 4678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Gentilini, E. Somigliana, P. Vigano, M. Vignali, M. Busacca, and A. M. Di Blasio The vascular endothelial growth factor +405G>C polymorphism in endometriosis Hum. Reprod., January 1, 2008; 23(1): 211 - 215. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||