Mol. Hum. Reprod. Advance Access originally published online on March 25, 2004
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Molecular Human Reproduction, Vol. 10, No. 6, pp. 461-466, 2004
© European Society of Human Reproduction and Embryology 2004
Comparative genomic hybridization and karyotyping of human embryonic stem cells reveals the occurrence of an isodicentric X chromosome after long-term cultivation
1Department of Clinical Sciences, Division of Obstetrics and Gynaecology, Karolinska Institutet, Karolinska University Hospital, Huddinge, S-141 86 Stockholm and 2Department of Molecular Medicine, Karolinska Institutet, Karolinska University Hospital, Solna, S-171 76 Stockholm, Sweden
3 To whom correspondence should be addressed. e-mail: jose.inzunza{at}mednut.ki.se
Human embryonic stem (hES) cells are important research tools in studies of the physiology of early tissue differentiation. In addition, prospects are high regarding the use of these cells for successful cell transplantation. However, one concern has been that cultivation of these cells over many passages might induce chromosomal changes. It is thus important to investigate these cell lines, and check that a normal chromosomal content is retained even during long-term in vitro culture. Comparative genomic hybridization (CGH) was used to analyse three hES cell lines derived in our laboratory and cultured continuously for 30–42 weeks, comprising 35–39 cell passages. CGH could be successfully performed in 48 out of a total of 50 isolated single cells (96%). All three lines (HS181, HS235 and HS237) were shown to have a normal chromosomal content when analysed by both single cell CGH and by karyotyping up to passages 39, 39 and 35 respectively. No aneuploidies or larger deletions or amplifications were detected, and they were female (46,XX). However, HS237 was reanalysed at passage 61, and at that point an aberrant X chromosome was detected by karyotyping. The aberration was confirmed and characterized by single cell CGH and fluorescence in situ hybridization analysis, 46,X,idic(X)(q21). Thus, chromosomal aberrations may occur over time in stem cell lines, and continuous analysis of these cells during cultivation is crucial. Single cell CGH is a method that can be used for continuous analysis of the hES cell lines during cultivation, in order to detect chromosome imbalance.
Key words: chromosome/comparative genomic hybridization/culture/human embryonic stem cells
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Strom, J. Inzunza, K.-H. Grinnemo, K. Holmberg, E. Matilainen, A.-M. Stromberg, E. Blennow, and O. Hovatta Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines Hum. Reprod., December 1, 2007; 22(12): 3051 - 3058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Allegrucci and L.E. Young Differences between human embryonic stem cell lines Hum. Reprod. Update, March 1, 2007; 13(2): 103 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Enver, S. Soneji, C. Joshi, J. Brown, F. Iborra, T. Orntoft, T. Thykjaer, E. Maltby, K. Smith, R. A. Dawud, et al. Cellular differentiation hierarchies in normal and culture-adapted human embryonic stem cells Hum. Mol. Genet., November 1, 2005; 14(21): 3129 - 3140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Stojkovic, M. Lako, T. Strachan, and A. Murdoch Derivation, growth and applications of human embryonic stem cells Reproduction, September 1, 2004; 128(3): 259 - 267. [Abstract] [Full Text] [PDF]
|
|