Mol. Hum. Reprod. Advance Access originally published online on June 13, 2005
Molecular Human Reproduction 2005 11(6):451-457; doi:10.1093/molehr/gah181
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Divergence of natural killer cell receptor and related molecule in the decidua from sporadic miscarriage with normal chromosome karyotype
1Department of Obstetrics and Gynecology, 3Department of Public Heath, Hokkaido University Graduate School of Medicine, and 2Division of Immunobiology, Research Section of Pathophysiology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan
4 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Hokkaido University Graduate School of Medicine, Kita-ku N15 W7, Sapporo 060-8638, Japan. Email: yhideto{at}med.hokudai.ac.jp
The aim of this cohort study was to investigate immunophenotypic characteristics of natural killer (NK) cells by assessing specific molecules expressed in the decidua of sporadic miscarriages and induced abortions. The deciduae were obtained from 29 consecutively seen women whose pregnancies ended in first trimester miscarriages (MS), and the fetal chromosome karyotype of these MS was analysed. Additionally, 13 deciduae were obtained from induced abortion (IA) with informed consent. The expression of perforin, CD94, CD161, CD158a, CD158b, CD244 on CD3–CD56+NK cells, and perforin on CD3+CD8+ T cells was analysed by flow cytometry. The CD158a (mean±SD, 26.2±14.7%) and CD94 (50.2±25.7%) expressions in MS with normal chromosome karyotype (MSNK; n=11) were significantly decreased as compared with those (41.5±19.5%, 71.4±20.4%) in MS with abnormal karyotype (MSAK; n=18) and those (44.3±21.9%, 80.8±17.5%) in IA (n=13). Conversely, the perforin expression on CD3–CD8–CD56+NK cells (76.3±11.0%) and CD3+CD8+T cells (30.6±9.2%) in MSNK was significantly increased as compared with those (66.8±16.6%, 23.6±8.7%) in MSAK and those (62.9±11.6%, 19.7±8.1%) in IA. A positive correlation between CD94 and CD158a expressions on NK cells, negative correlations between CD94 on NK cells and perforin on NK cells/T cells, and between CD158a on NK cells and perforin on T cells were found in the decidua. A divergence of NK cell repertoire in the decidua might be related to aetiology of sporadic MSNK.
Key words: KIR/natural killer cell/pregnancy/recurrent miscarriage/recurrent spontaneous abortion
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