Mol. Hum. Reprod. Advance Access originally published online on October 4, 2006
Molecular Human Reproduction 2006 12(12):749-754; doi:10.1093/molehr/gal082
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Deficient expression of monoamine oxidase A in the endometrium is associated with implantation failure in women participating as recipients in oocyte donation
1Laboratorio de Inmunología de la Reproducción, Universidad de Santiago de Chile, 2Instituto Chileno de Medicina Reproductiva, 3Millenium Institute for Fundamental and Applied Biology, 4Clínica Las Condes, Santiago, Chile and 5Laboratory of Molecular Technology, SAIC-Frederick, National Cancer Institute at Frederick, Frederick, MD, USA
6 To whom correspondence should be addressed at: Alameda 3363, Casilla 40, Correo 33, Santiago, Chile. E-mail: lvelasqu{at}lauca.usach.cl
Successful implantation depends both on the quality of the embryo and on the endometrial receptivity. The latter depends on progesterone-induced changes in gene expression, a process that has been characterized by microarray analysis. One of the genes whose transcription appears to be enhanced during the receptive period is monoamine oxidase A (MAO-A). Our first objective was to confirm the increased expression of MAO-A in the endometrium during the receptive phase of spontaneous normal cycles using real time PCR and immunofluorescence. The second objective was to examine the endometrial expression of MAO-A during the receptive phase induced by exogenous estradiol (E2) and progesterone in patients whose endometrium was shown to have been either receptive or non-receptive to embryo implantation in repeated cycles of oocyte donation. Results showed that MAO-A transcript levels increased between the pre-receptive (LH+3) and receptive phase (LH+7) in all spontaneous cycles examined, with a median increase of 25-fold. Immunofluorescent labelling demonstrated MAO-A localization to the glandular and luminal epithelium with an increasing positive score between LH+3 and LH+7. Conversely, prior failure of embryo implantation was associated with a 29-fold decrease in MAO-A mRNA levels and a substantial reduction in MAO-A protein immunofluorescent label score. These results show a strong association between endometrial receptivity and MAO-A expression in the endometrial epithelium, suggesting an important role for this enzyme in normal implantation.
Key words: monoamine oxidase A/endometrium/implantation failure/implantation window/oocyte donation
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. A. Bersinger, D. M. Wunder, M. H. Birkhauser, and M. D. Mueller Gene expression in cultured endometrium from women with different outcomes following IVF Mol. Hum. Reprod., August 1, 2008; 14(8): 475 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Takano, Z. Lu, T. Goto, L. Fusi, J. Higham, J. Francis, A. Withey, J. Hardt, B. Cloke, A. V. Stavropoulou, et al. Transcriptional Cross Talk between the Forkhead Transcription Factor Forkhead Box O1A and the Progesterone Receptor Coordinates Cell Cycle Regulation and Differentiation in Human Endometrial Stromal Cells Mol. Endocrinol., October 1, 2007; 21(10): 2334 - 2349. [Abstract] [Full Text] [PDF]
|
|