Molecular Human Reproduction, Vol 3, 15-19, Copyright © 1997 by Oxford University Press
SM Wykes, DW Visscher and SA Krawetz
Mammalian spermiogenesis is marked by the morphological and functional
differentiation of round haploid spermatids into mature spermatozoa. A
molecular restructuring of the chromatin accompanies this process
facilitated by the transition proteins and protamines which compact and
condense the genetic material within the developing spermatid. Previous
studies from this laboratory have demonstrated that human protamines PRM1,
PRM2 and transition protein TNP2 transcripts are associated with round and
elongating spermatids. Extending this investigation, we examined the
occurrence of these transcripts in mature spermatozoa by in-situ
hybridization analysis using [35S]-labelled cRNA probes. These results
demonstrate that PRM1, PRM2 and TNP2 haploid-specific transcripts are
present in mature spermatozoa. Quantitative analysis of the localized
signal also indicates that the PRM1, PRM2 and TNP2 transcripts persist at a
similar ratio to that previously described for these transcripts in human
testes, i.e. PRM2 > PRM1 approximately equal to TNP2. The persistence of
these transcripts in mature spermatozoa warrants further investigation.
JOURNAL ARTICLE
Haploid transcripts persist in mature human spermatozoa
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  W. Yan, K. Morozumi, J. Zhang, S. Ro, C. Park, and R. Yanagimachi Birth of Mice after Intracytoplasmic Injection of Single Purified Sperm Nuclei and Detection of Messenger RNAs and MicroRNAs in the Sperm Nuclei Biol Reprod, May 1, 2008; 78(5): 896 - 902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Gilbert, N. Bissonnette, G. Boissonneault, M. Vallee, and C. Robert A molecular analysis of the population of mRNA in bovine spermatozoa Reproduction, June 1, 2007; 133(6): 1073 - 1086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Yatsenko, A. Roy, R. Chen, L. Ma, L. J. Murthy, W. Yan, D. J. Lamb, and M. M. Matzuk Non-invasive genetic diagnosis of male infertility using spermatozoal RNA: KLHL10mutations in oligozoospermic patients impair homodimerization Hum. Mol. Genet., December 1, 2006; 15(23): 3411 - 3419. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Agirregoitia, A. Valdivia, A. Carracedo, L. Casis, J. Gil, N. Subiran, C. Ochoa, and J. Irazusta Expression and Localization of {delta}-, {kappa}-, and {micro}-Opioid Receptors in Human Spermatozoa and Implications for Sperm Motility J. Clin. Endocrinol. Metab., December 1, 2006; 91(12): 4969 - 4975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Miller and G.C. Ostermeier Towards a better understanding of RNA carriage by ejaculate spermatozoa Hum. Reprod. Update, November 1, 2006; 12(6): 757 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Gur and H. Breitbart Mammalian sperm translate nuclear-encoded proteins by mitochondrial-type ribosomes Genes & Dev., February 15, 2006; 20(4): 411 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V.W. Aoki, L. Liu, and D.T. Carrell A novel mechanism of protamine expression deregulation highlighted by abnormal protamine transcript retention in infertile human males with sperm protamine deficiency Mol. Hum. Reprod., January 1, 2006; 12(1): 41 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.P. Dadoune, A. Pawlak, M.F. Alfonsi, and J.P. Siffroi Identification of transcripts by macroarrays, RT-PCR and in situ hybridization in human ejaculate spermatozoa Mol. Hum. Reprod., February 1, 2005; 11(2): 133 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Januchowski, A. K. Breborowicz, H. Ofori, P. Jedrzejczak, L. Pawelczyk, and P. P. Jagodzinski Detection of a Short CCR5 Messenger RNA Isoform in Human Spermatozoa J Androl, September 1, 2004; 25(5): 757 - 760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Steger, L. Fink, K. Failing, R. M. Bohle, S. Kliesch, W. Weidner, and M. Bergmann Decreased protamine-1 transcript levels in testes from infertile men Mol. Hum. Reprod., June 1, 2003; 9(6): 331 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. van Biljon, S. Wykes, S. Scherer, S.A. Krawetz, and J. Hapgood Type II Gonadotropin-Releasing Hormone Receptor Transcripts in Human Sperm Biol Reprod, December 1, 2002; 67(6): 1741 - 1749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ziyyat and A. Lefevre Differential gene expression in pre-implantation embryos from mouse oocytes injected with round spermatids or spermatozoa Hum. Reprod., July 1, 2001; 16(7): 1449 - 1456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Steger, K. Failing, T. Klonisch, H. M. Behre, M. Manning, W. Weidner, L. Hertle, M. Bergmann, and S. Kliesch Round spermatids from infertile men exhibit decreased protamine-1 and -2 mRNA Hum. Reprod., April 1, 2001; 16(4): 709 - 716. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Steger, K. Pauls, T. Klonisch, F. E. Franke, and M. Bergmann Expression of protamine-1 and -2 mRNA during human spermiogenesis Mol. Hum. Reprod., March 1, 2000; 6(3): 219 - 225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. O. Goodwin, D. S. Karabinus, R. G. Pergolizzi, and S. Benoff L-type voltage-dependent calcium channel {alpha}-1C subunit mRNA is present in ejaculated human spermatozoa Mol. Hum. Reprod., February 1, 2000; 6(2): 127 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bahadur and D. Ralph Gonadal tissue cryopreservation in boys with paediatric cancers Hum. Reprod., January 1, 1999; 14(1): 11 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Rovescalli, M. Cinquanta, J. Ferrante, C. A. Kozak, and M. Nirenberg The mouse Nkx-1.2 homeobox gene: Alternative RNA splicing at canonical and noncanonical splice sites PNAS, February 29, 2000; 97(5): 1982 - 1987. [Abstract] [Full Text] [PDF]
|
|