Molecular Human Reproduction, Vol 3, 21-26, Copyright © 1997 by Oxford University Press
HC Liu, ZY He, C Mele, M Damario, O Davis and Z Rosenwaks
To investigate the presence of messenger RNA (mRNA) encoding insulin- like
growth factor binding proteins (IGFBP) in human secretory endometrial
stromal cells cultured in vitro, total cellular mRNA and protein extracted
from cells treated with various hormones were detected and identified by
Northern and Western blotting techniques respectively. Northern blot
analysis detected 1.4 and 2.5 kilobase (kb) mRNA transcripts for IGFBP-2
and IGFBP-3 respectively, in both control and progestin-treated human
endometrial stromal cells in vitro. However, the 1.5 kb mRNA transcript of
IGFBP-1 was detected only in progestin-treated cells but not in the
controls. Progestin alone markedly stimulated cellular BP-1 protein and
mRNA, but only moderately stimulated cellular IGFBP-2 and IGFBP-3 protein
mRNA in a dose- dependent fashion. Adding relaxin at the same time as
progestin further enhanced the stimulatory effects of progesterone.
Oestradiol had a stimulatory effect on cellular IGFBP-2 mRNA, but had an
inhibitory effect on protein and mRNA of IGFBP-3, also in a dose-dependent
fashion. In general, for each specific binding protein, the amount of
cellular mRNA correlated well with the amount of cellular protein.
Therefore, IGFBP protein and mRNA transcript in human secretory endometrial
stromal cells appears to be under hormonal influence. These hormones may
control the synthesis of IGFBPs at the transcription rather than the
translation level.
JOURNAL ARTICLE
Hormonal regulation of expression of messenger RNA encoding insulin- like growth factor binding proteins in human endometrial stromal cells cultured in vitro
Department of Obstetrics and Gynecology, Cornell University Medical College, New York, NY 10021, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. J. Gunter, D. R. Hoover, H. Yu, S. Wassertheil-Smoller, J. E. Manson, J. Li, T. G. Harris, T. E. Rohan, X. Xue, G. Y.F. Ho, et al. A Prospective Evaluation of Insulin and Insulin-like Growth Factor-I as Risk Factors for Endometrial Cancer Cancer Epidemiol. Biomarkers Prev., April 1, 2008; 17(4): 921 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. V. Lacey Jr., N. Potischman, M. P. Madigan, M. L. Berman, R. Mortel, L. B. Twiggs, R. J. Barrett, G. D. Wilbanks, J. R. Lurain, C.-M. Fillmore, et al. Insulin-Like Growth Factors, Insulin-Like Growth Factor-Binding Proteins, and Endometrial Cancer in Postmenopausal Women: Results from a U.S. Case-Control Study Cancer Epidemiol. Biomarkers Prev., April 1, 2004; 13(4): 607 - 612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kaaks, A. Lukanova, and M. S. Kurzer Obesity, Endogenous Hormones, and Endometrial Cancer Risk: A Synthetic Review Cancer Epidemiol. Biomarkers Prev., December 1, 2002; 11(12): 1531 - 1543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hombach-Klonisch, S. Seeger, G. Tscheudschilsuren, J. Buchmann, B. Huppertz, G. Seliger, B. Fischer, and T. Klonisch Cellular localization of human relaxin-like factor in the cyclic endometrium and placenta Mol. Hum. Reprod., April 1, 2001; 7(4): 349 - 356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Fowler, G. Albaiges, C. Lees, J. Jones, K. Nicolaides, and J. Miell The role of insulin-like growth factor binding protein-1 phosphoisoforms in pregnancies with impaired placental function identified by Doppler ultrasound Hum. Reprod., November 1, 1999; 14(11): 2881 - 2885. [Abstract] [Full Text] [PDF]
|
|