Molecular Human Reproduction, Vol 3, 47-54, Copyright © 1997 by Oxford University Press
R Golan, L Shochat, R Weissenberg, Y Soffer, Z Marcus, Y Oschry and LM Lewin
The quality of sperm chromatin is an important factor in fertilization and
is especially critical where one spermatozoon is artificially selected for
fertilizing an egg (as in intracytoplasmic sperm injection). In this study,
flow cytometry after staining of human spermatozoa with Acridine Orange was
used to study chromatin structure. A method is described for estimating the
percentage of cells in a human sperm sample that have completed epididymal
maturation in regard to chromatin condensation. Of the 121 samples of the
semen that were examined, nine contained a higher percentage of
hypocondensed spermatozoa and six samples contained elevated amounts of
hypercondensed spermatozoa. In addition to aberrancies in chromatin
condensation other defects showed up as satellite populations of
spermatozoa with higher than normal ratios of red/green fluorescence after
Acridine Orange staining. Such defects were found in 15 semen samples. The
use of swim-up and Percoll gradient centrifugation methods was shown to
improve the percentage of spermatozoa with normal chromatin structure in
some samples with poor initial quality.
JOURNAL ARTICLE
Evaluation of chromatin condensation in human spermatozoa: a flow cytometric assay using acridine orange staining
Department of Clinical Biochemistry, Sackler Medical School, Tel Aviv University, Ramat Aviv, Israel.
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