Molecular Human Reproduction, Vol 3, 459-466, Copyright © 1997 by Oxford University Press
R Ivell, M Balvers, R Domagalski, H Ungefroren, N Hunt and W Schulze
The complete protein-coding region of the human relaxin-like factor (RLF;
formerly Ley-I-L) was cloned by reverse transcription-polymerase chain
reaction from human testis and subcloned into a bacterial expression
plasmid for the production of recombinant human RLF in Escherichia coli.
Polyclonal antibodies were raised against the recombinant RLF, as well as
against a peptide epitope from the B-domain of the RLF polypeptide.
Antibodies were used for immunohistochemistry of Bouin-fixed,
paraffin-embedded samples of human testis tissues. Specific
immunoreactivity was located exclusively in the Leydig cells with a
consistent high intensity of staining, showing similar spatial distribution
to other Leydig cell markers, such as the luteinizing hormone (LH) receptor
and 3 beta-hydroxysteroid dehydrogenase (3 beta- HSD), and to the pattern
of RLF mRNA shown by in-situ transcript hybridization. In biopsy samples
from patients with severe disturbances of spermatogenesis, RLF staining
intensity was consistently high in all cases, unlike staining for 3
beta-HSD which varied considerably between patients. Immunostaining for RLF
would thus appear to offer an interesting new marker for Leydig cells in
human testis samples.
JOURNAL ARTICLE
Relaxin-like factor: a highly specific and constitutive new marker for Leydig cells in the human testis
Institute for Hormone and Fertility Research, University of Hamburg, Germany.
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