Molecular Human Reproduction, Vol 4, 757-762, Copyright © 1998 by Oxford University Press
J Tesarik, E Greco, P Cohen-Bacrie and C Mendoza
Germ cell apoptosis was evaluated in 11 men suffering from nonobstructive
azoospermia and enrolled in a spermatid conception programme. In six of
these patients, round spermatids (Sa stage) were the most advanced
spermatogenic cells recovered from testicular biopsy samples. This
condition is referred to as complete spermiogenesis failure. In the
remaining five men, a few late elongated spermatids (Sd stage) were
unexpectedly found in the testicular biopsy samples on the day of
treatment. This condition is referred to as incomplete spermiogenesis
failure. Germ cell apoptosis in both groups of patients was examined by
analysing cell smears prepared from mechanically disintegrated testicular
tissues using terminal deoxyribonucleotidyl transferase-mediated dUTP
nick-end labelling (TUNEL), which detects apoptosis-specific DNA
fragmentation, and annexin-V binding, detecting apoptosis-related
translocation of plasma membrane phosphatidylserine to the membrane's outer
surface. Both methods were combined, in double- fluorescence labelling
preparations, with immunocytochemical detection of proacrosin, a specific
germline marker. Patients with complete spermiogenesis failure had
significantly higher frequencies of primary spermatocytes and round
spermatids carrying the apoptosis-specific DNA damage in comparison with
patients with incomplete spermiogenesis failure. Surprisingly,
apoptosis-related phosphatidylserine externalization occurs rarely until
the advanced stages of spermiogenesis. Since externalized
phosphatidylserine is expected to be involved in the recognition of
apoptotic cells by phagocytes, apoptotic spermatocytes and round spermatids
may not be removed easily by phagocytosis. The high frequency of DNA damage
in round spermatids from patients with complete spermiogenesis failure
explains the low success rates of spermatid conception in these cases. The
evaluation of apoptosis can help predict success rates of spermatid
conception.
JOURNAL ARTICLE
Germ cell apoptosis in men with complete and incomplete spermiogenesis failure
Laboratoire d'Eylau, Paris, France.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y.-J. Li, T.-B. Song, Y.-Y. Cai, J.-S. Zhou, X. Song, X. Zhao, and X.-L. Wu Bisphenol A Exposure Induces Apoptosis and Upregulation of Fas/FasL and Caspase-3 Expression in the Testes of Mice Toxicol. Sci., April 1, 2009; 108(2): 427 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Marchiani, L. Tamburrino, A. Maoggi, G.B. Vannelli, G. Forti, E. Baldi, and M. Muratori Characterization of M540 bodies in human semen: evidence that they are apoptotic bodies Mol. Hum. Reprod., September 1, 2007; 13(9): 621 - 631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Smith, H. Kaune, D. Parodi, M. Madariaga, R. Rios, I. Morales, and A. Castro Increased sperm DNA damage in patients with varicocele: relationship with seminal oxidative stress Hum. Reprod., April 1, 2006; 21(4): 986 - 993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Brugnon, E. Van Assche, G. Verheyen, B. Sion, D. Boucher, J.L. Pouly, L. Janny, P. Devroey, I. Liebaers, and A. Van Steirteghem Study of two markers of apoptosis and meiotic segregation in ejaculated sperm of chromosomal translocation carrier patients Hum. Reprod., March 1, 2006; 21(3): 685 - 693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Lee, K.-S. Kim, Y. H. Yang, H. S. Oh, S. H. Lee, T. G. Chung, J. H. Cho, H. J. Kim, T. K. Yoon, and K. Y. Cha Isolation of male germ stem cell-like cells from testicular tissue of non-obstructive azoospermic patients and differentiation into haploid male germ cells in vitro Hum. Reprod., February 1, 2006; 21(2): 471 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Martin, O. Sabido, P. Durand, and R. Levy Phosphatidylserine externalization in human sperm induced by calcium ionophore A23187: relationship with apoptosis, membrane scrambling and the acrosome reaction Hum. Reprod., December 1, 2005; 20(12): 3459 - 3468. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sofikitis, E. Pappas, A. Kawatani, D. Baltogiannis, D. Loutradis, N. Kanakas, D. Giannakis, F. Dimitriadis, K. Tsoukanelis, I. Georgiou, et al. Efforts to create an artificial testis: culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment Hum. Reprod. Update, May 1, 2005; 11(3): 229 - 259. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q Bian, L C Xu, S L Wang, Y K Xia, L F Tan, J F Chen, L Song, H C Chang, and X R Wang Study on the relation between occupational fenvalerate exposure and spermatozoa DNA damage of pesticide factory workers Occup. Environ. Med., December 1, 2004; 61(12): 999 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tesarik, F. Ubaldi, L. Rienzi, F. Martinez, M. Iacobelli, C. Mendoza, and E. Greco Caspase-dependent and -independent DNA fragmentation in Sertoli and germ cells from men with primary testicular failure: relationship with histological diagnosis Hum. Reprod., February 1, 2004; 19(2): 254 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Moustafa, R. K. Sharma, J. Thornton, E. Mascha, M. A. Abdel-Hafez, A. J. Thomas, and A. Agarwal Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility Hum. Reprod., January 1, 2004; 19(1): 129 - 138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Erkkila, L. Suomalainen, M. Wikstrom, M. Parvinen, and L. Dunkel Chemical Anoxia Delays Germ Cell Apoptosis in the Human Testis Biol Reprod, August 1, 2003; 69(2): 617 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Tesarik and C. Mendoza Using the Male Gamete for Assisted Reproduction: Past, Present, and Future J Androl, May 1, 2003; 24(3): 317 - 328. [Full Text] [PDF]
|
|
|
|
|
|
U. Paasch, S. Grunewald, G. Fitzl, and H.-J. Glander Deterioration of Plasma Membrane Is Associated With Activated Caspases in Human Spermatozoa J Androl, March 1, 2003; 24(2): 246 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tesarik, F. Martinez, L. Rienzi, M. Iacobelli, F. Ubaldi, C. Mendoza, and E. Greco In-vitro effects of FSH and testosterone withdrawal on caspase activation and DNA fragmentation in different cell types of human seminiferous epithelium Hum. Reprod., July 1, 2002; 17(7): 1811 - 1819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-M. Shen, J. Dai, S.-E. Chia, A. Lim, and C.-N. Ong Detection of apoptotic alterations in sperm in subfertile patients and their correlations with sperm quality Hum. Reprod., May 1, 2002; 17(5): 1266 - 1273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Coskun, A. Tbakhi, K. A. Jaroudi, M. Uzumcu, T. A. Merdad, and K. A. Al-Hussein Flow cytometric ploidy analysis of testicular biopsies from sperm-negative wet preparations Hum. Reprod., April 1, 2002; 17(4): 977 - 983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sakkas, O. Moffatt, G. C. Manicardi, E. Mariethoz, N. Tarozzi, and D. Bizzaro Nature of DNA Damage in Ejaculated Human Spermatozoa and the Possible Involvement of Apoptosis Biol Reprod, April 1, 2002; 66(4): 1061 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Francavilla, P. D'Abrizio, G. Cordeschi, F. Pelliccione, S. Necozione, S. Ulisse, G. Properzi, and F. Francavilla Fas expression correlates with human germ cell degeneration in meiotic and post-meiotic arrest of spermatogenesis Mol. Hum. Reprod., March 1, 2002; 8(3): 213 - 220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tesarik, E. Greco, and C. Mendoza Assisted reproduction with in-vitro-cultured testicular spermatozoa in cases of severe germ cell apoptosis: a pilot study Hum. Reprod., December 1, 2001; 16(12): 2640 - 2645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cremades, M. Sousa, R. Bernabeu, and A. Barros Developmental potential of elongating and elongated spermatids obtained after in-vitro maturation of isolated round spermatids Hum. Reprod., September 1, 2001; 16(9): 1938 - 1944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ramos and A. M. M. Wetzels Low rates of DNA fragmentation in selected motile human spermatozoa assessed by the TUNEL assay Hum. Reprod., August 1, 2001; 16(8): 1703 - 1707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ziyyat and A. Lefevre Differential gene expression in pre-implantation embryos from mouse oocytes injected with round spermatids or spermatozoa Hum. Reprod., July 1, 2001; 16(7): 1449 - 1456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Emiliani, M. Van den Bergh, A.-S. Vannin, J. Biramane, M. Verdoodt, and Y. Englert Evidence of reduced single-stranded testicular sperm DNA from obstructive azoospermic men after 3 days of in-vitro culture Hum. Reprod., June 1, 2001; 16(6): 1200 - 1203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tesarik, C. Mendoza, and E. Greco The effect of FSH on male germ cell survival and differentiation in vitro is mimicked by pentoxifylline but not insulin Mol. Hum. Reprod., October 1, 2000; 6(10): 877 - 881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Balaban, B. Urman, A. Isiklar, C. Alatas, S. Aksoy, R. Mercan, and A. Nuhoglu Progression to the blastocyst stage of embryos derived from testicular round spermatids Hum. Reprod., June 1, 2000; 15(6): 1377 - 1382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bahadur, R. Chatterjee, and D. Ralph Testicular tissue cryopreservation in boys. Ethical and legal issues: Case report Hum. Reprod., June 1, 2000; 15(6): 1416 - 1420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Tsai, T. Takeuchi, J.M. Bedford, M. M. Reis, Z. Rosenwaks, and G. D. Palermo Alternative sources of gametes: reality or science fiction? Hum. Reprod., May 1, 2000; 15(5): 988 - 998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Prapas, A. Chatziparasidou, P. Vanderzwalmen, M. Nijs, N. Prapas, B. Lejeune, G. Vlassis, and R. Schoysman Spermatid injection: Reconsidering spermatid injection Hum. Reprod., September 1, 1999; 14(9): 2186 - 2188. [Full Text] [PDF]
|
|
|
|
|
|
M. Sousa, A. Barros, K. Takahashi, C. Oliveira, J. Silva, and J. Tesarik Clinical efficacy of spermatid conception: analysis using a new spermatid classification scheme Hum. Reprod., May 1, 1999; 14(5): 1279 - 1286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ogura, K. Inoue, and J. Matsuda Mouse spermatid nuclei can support full term development after premature chromosome condensation within mature oocytes Hum. Reprod., May 1, 1999; 14(5): 1294 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Jurisicova, S. Lopes, J. Meriano, L. Oppedisano, R. F. Casper, and S. Varmuza DNA damage in round spermatids of mice with a targeted disruption of the Pp1c{gamma} gene and in testicular biopsies of patients with non-obstructive azoospermia Mol. Hum. Reprod., April 1, 1999; 5(4): 323 - 330. [Abstract] [Full Text] [PDF]
|
|