Molecular Human Reproduction, Vol. 5, No. 12, 1135-1140,
December 1999
© 1999 European Society of Human Reproduction and Embryology
Molecular events in the uterus |
Interleukin-8 expression in endometrial stromal cells is regulated by integrin-dependent cell adhesion
Department of Obstetrics and Gynecology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520–8063 USA
Abstract
Concentrations of interleukin (IL)-8, a potent chemotactic factor produced by many cell types, are elevated in the peritoneal fluid of women with endometriosis. We investigated whether endometrial stromal cell (ESC) adhesion induces the expression of IL-8 and if this process is integrin-mediated. ESCs were plated onto culture dishes coated with various extracellular matrix (ECM) substrates, such as fibronectin, laminin, collagen IV, and poly-L-lysine, or mouse anti-human integrin ß1, and ß2 monoclonal antibodies. IL-8 expression was induced by adherence of ESCs to fibronectin or collagen IV, but not to poly-L-lysine, a non-integrin-dependent adhesion matrix. Engagement of ß1-containing integrins was associated with ESC adhesion and resulted in up-regulation of IL-8 mRNA expression and protein secretion. Disruption of the actin cytoskeleton by treating ESC with cytochalasin D completely blocked the increase of IL-8 that was induced in response to integrin activation. These findings indicate a novel mechanism of IL-8 regulation; cell adhesion to ECM is an important event that leads to stimulation of IL-8 expression, and this process is mediated by integrins.
adhesion/endometrium/extracellular matrix/integrins/interleukin-8
Notes
1 Current address: IVI-Madrid, C/Santiago de Compostela 88, 28035 Madrid, Spain
2 To whom correspondence should be addressed
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