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Molecular Human Reproduction, Vol. 5, No. 4, 353-357, April 1999
© 1999 European Society of Human Reproduction and Embryology

Expression of inducible nitric oxide synthase in human cultured endometrial stromal cells

Naoyuki Yoshiki1, Toshiro Kubota, Yukie Matsumoto and Takeshi Aso

Department of Obstetrics and Gynaecology, Faculty of Medicine, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan

The aim of the present study was to investigate the expression of mRNA and protein for inducible nitric oxide synthase (iNOS) in human cultured endometrial stromal cells. The endometrial stromal cells were cultured in the absence or the presence of cytokines such as interleukin (IL)-1ß and interferon (IFN)-{gamma}, which are inherently detectable in the endometrium. Nested reverse transcription–polymerase chain reaction detected iNOS mRNA in stromal cells cultured without cytokines. Northern blot analysis failed to detect iNOS mRNA in stromal cells cultured for 9 h in the absence of cytokines or in the presence of IL-1ß (10–100 ng/ml) or IFN-{gamma} (10–1000 U/ml) alone, but could detect iNOS mRNA cultured in combinations of IL-1ß and IFN-{gamma}. The concentrations of iNOS mRNA were increased as early as 3 h after the addition of the cytokine combination and persisted for up to 36 h. Western blot analysis demonstrated iNOS protein in stromal cells cultured for 12 h with combined IL-1ß and IFN-{gamma}. These results raise the possibility that nitric oxide locally synthesized by iNOS may be involved in the control of endometrial functions.

endometrial stromal cells/inducible nitric oxide synthase/interferon-{gamma}/interleukin-1ß

1 To whom correspondence should be addressed


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