Molecular Human Reproduction, Vol. 6, No. 12, 1069-1078,
December 2000
© 2000 European Society of Human Reproduction and Embryology
Testis and spermatogenesis |
Expression and characterization of the human YWK-II gene, encoding a sperm membrane protein related to the Alzheimer ßA4-amyloid precursor protein
1 National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Faculty of Basic Medicine, Peking Union Medical College, 5 Dong Dan 3 Tiao, Beijing 100005, 2 Shanghai Institute of Cell Biology, Academia Sinica, Shanghai 200031, China, and 3 Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10021, USA
Abstract
The YWK-II cDNA, RSD-2, encoding a sperm membrane protein was isolated from a rat testis cDNA expression library. Using the RSD-2 insert in combination with rapid amplification of cDNA ends (RACE), the corresponding human gene was isolated from a human testis cDNA expression library. The human testis cDNA, HSD-2, is 3654 bp in length and contains an open reading frame of 763 codons. Hydropathicity analysis showed that the deduced polypeptide is a single strand transmembrane protein. The deduced polypeptide has partial homology with the amyloid precursor protein (APP) and high homology with the amyloid precursor homologue, APLP2/APPH. The YWK-II gene was mapped and assigned to human chromosome locus: 11q24-25. Northern blotting of various human tissue RNAs using the HSD-2 cDNA as a probe showed that the gene is transcribed ubiquitously. The cytoplasmic domain of HSD-2 was expressed in Escherichia coli. In-vitro studies showed that the recombinant polypeptide bound to a GTP-binding protein (Go) and was phosphorylated by protein kinase C and cdc2 kinase. In mammalian F11 cells, the recombinant polypeptide was found to be coupled to Go. Thus, the YWK-II component has the characteristics of a Go-coupled receptor and may be involved in Go-mediated signal transduction pathway. Protein kinase C and cdc2 kinase may regulate this pathway in spermatozoa by phosphorylating the cytoplasmic domain of the YWK-II component.
cell signalling/G proteins/protein kinase/sperm membrane protein/YWK-II
Notes
4 To whom correspondence should be addressed. E-mail: wanglf{at}cdm.imicams.ac.cn
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Yin, S. Ouyang, W. Xu, X. Zhang, K. L. Fok, H. Y. Wong, J. Zhang, X. Qiu, S. Miao, H. C. Chan, et al. YWK-II protein as a novel Go-coupled receptor for Mullerian inhibiting substance in cell survival J. Cell Sci., May 1, 2007; 120(9): 1521 - 1528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. FARDILHA, S. I. VIEIRA, A. BARROS, M. SOUSA, O. A.B. DA CRUZ E SILVA, and E. F. DA CRUZ E SILVA Differential Distribution of Alzheimer's Amyloid Precursor Protein Family Variants in Human Sperm , January 1, 2007; 1096(1): 196 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hashimoto, T. Niikura, T. Chiba, E. Tsukamoto, H. Kadowaki, H. Nishitoh, Y. Yamagishi, M. Ishizaka, M. Yamada, M. Nawa, et al. The Cytoplasmic Domain of Alzheimer's Amyloid-{beta} Protein Precursor Causes Sustained Apoptosis Signal-Regulating Kinase 1/c-Jun NH2-Terminal Kinase-Mediated Neurotoxic Signal via Dimerization J. Pharmacol. Exp. Ther., September 1, 2003; 306(3): 889 - 902. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hashimoto, T. Niikura, Y. Ito, H. Sudo, M. Hata, E. Arakawa, Y. Abe, Y. Kita, and I. Nishimoto Detailed Characterization of Neuroprotection by a Rescue Factor Humanin against Various Alzheimer's Disease-Relevant Insults J. Neurosci., December 1, 2001; 21(23): 9235 - 9245. [Abstract] [Full Text] [PDF]
|
|