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Molecular Human Reproduction, Vol. 6, No. 3, 215-218, March 2000
© 2000 European Society of Human Reproduction and Embryology

Testis and spermatozoa

Prostatic origin of a zinc binding high molecular weight protein complex in human seminal plasma

L. Siciliano1, C. De Stefano2, M.F. Petroni1, A. Vivacqua1, V. Rago1 and A. Carpino1,3

1 Dipartimento di Biologia Cellulare, Università degli Studi della Calabria, 87030 Arcavacata di Rende, Cosenza, and 2 Centro Trattamento della Sterilità, Diagnostica Medica, 86100 Avellino, Italy

Abstract

The profile of the zinc ligand high molecular weight proteins was investigated in the seminal plasma of 55 normozoospermic subjects by size exclusion high performance liquid chromatography (HPLC). The proteins were recovered from Sephadex G-75 gel filtration of seminal plasma in three zinc-containing fractions which were then submitted to HPLC analysis. The results were, that in all the samples, the protein profiles showed two peaks with apparent molecular weight of ~660 and ~250 kDa. Dialysis experiments revealed that both ~660 and ~250 kDa proteins were able to uptake zinc against gradient indicating their zinc binding capacity. The HPLC analysis of the whole seminal plasma evidenced only the ~660 kDa protein complex as a single well quantifying peak, furthermore a positive correlation between its peak area and the seminal zinc values (P < 0.001) was observed. This suggested a prostatic origin of the ~660 kDa protein complex which was then confirmed by the seminal plasma HPLC analysis of a subject with agenesis of the Wolffian ducts. Finally the study demonstrated the presence of two zinc binding proteins, ~660 and ~250 kDa respectively, in human seminal plasma and the prostatic origin of the ~660 kDa.

high molecular weight proteins/human semen/prostatic secretion/protein profile/zinc ligands

Notes

3 To whom correspondence should be addressed


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