Molecular Human Reproduction, Vol. 8, No. 10, 930-933,
October 2002
© 2002 European Society of Human Reproduction and Embryology
Uterine physiology |
Expression of macrophage inflammatory protein-3
in an endometrial epithelial cell line, HHUA, and cultured human endometrial stromal cells
Department of Obstetrics and Gynecology, Oita Medical University, Hasama-machi, Oita 879-5593, Japan
It has been demonstrated that human endometrial epithelial cells (EEC) and stromal cells (ESC) produce a variety of chemokines in vivo and in vitro. To evaluate the expression of macrophage inflammatory protein (MIP)-3
in endometrial cells, the production of MIP-3 by an EEC line, HHUA, and cultured ESC stimulated with various inflammatory mediators was evaluated by ELISA. Unstimulated HHUA and ESC constitutively secreted MIP-3. Tumour necrosis factor- and interleukin-1ß significantly stimulated the secretion of MIP-3 by HHUA and ESC. Lipopolysaccharide also stimulated the secretion of MIP-3 by ESC, but not by HHUA. These results show that the concentration of MIP-3 in the endometrium is modulated by these inflammatory mediators. MIP-3 may contribute to the normal and pathological processes of human reproduction by regulating the trafficking of immature dendritic cells and memory T lymphocytes into the endometrium.
endometrium/interleukin-1ß/lipopolysaccharide/macrophage inflammatory protein-3/tumour necrosis factor-
1 To whom correspondence should be addressed. E-mail: nasu{at}oita-med.ac.jp
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