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Mol. Hum. Reprod. Advance Access published online on March 2, 2004
Molecular Human Reproduction, doi:10.1093/molehr/gah037
© 2004 by Oxford University Press
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Received September 22, 2003
Revised November 20, 2003
Accepted November 24, 2003

Article

Developmental stage-specific expression of Rbm suggests its involvement in early phases of spermatogenesis

Jungmin Lee 1, Jinpyo Hong 1, Euisu Kim 1, Kyungjin Kim 1, Soo Woong Kim 2, Hanumanthappa Krishnamurthy 3, Sanny S.W. Chung 3, Debra J. Wolgemuth 3, Kunsoo Rhee 4*

1 School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
2 Department of Urology, Seoul National University College of Medicine, Seoul 110-744, Korea
3 Columbia University College of Physicians and Surgeons, Departments of Genetics and Development and Obstetrics and Gynecology and The Institute of Human Nutrition, New York, NY 10032, USA
4 School of Biological Sciences, Seoul National University, Seoul 151-742, Korea

* To whom correspondence should be addressed. E-mail: rheek{at}snu.ac.kr.


  Abstract

Rbm is a male infertility gene located on the Y chromosome that is expressed in the testis. To investigate the specific events of spermatogenesis in which Rbm plays a role, the precise pattern of expression of Rbm in the mouse testis was determined. An antibody was generated against the Rbm protein and used to detect a single specific band of 43 kDa in size in mouse testicular lysates. In situ hybridization, immunoblot and immunohistochemistry analyses together indicated that Rbm was expressed in spermatogonia, preleptotene spermatocytes, late leptotene to early pachytene spermatocytes but not in mid-pachytene spermatocytes or subsequent stages of differentiation, including haploid germ cells. These observations suggest that Rbm functions in early but not later stages of male germ cell development.

Key Words: Key words: male infertility/Rbm/spermatogenesis


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