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Mol. Hum. Reprod. Advance Access published online on July 8, 2004
Molecular Human Reproduction, doi:10.1093/molehr/gah083
© 2004 by Oxford University Press
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Received May 17, 2004
Accepted June 9, 2004

Article

Basic fibroblast growth factor expression in isolated small human ovarian follicles

J.H. Quennell 1, J-A.L. Stanton 1, P.R. Hurst 1*

1 Department of Anatomy and Structural Biology, School of Medical Sciences, University of Otago, P.O.Box 56, Dunedin, 9001,New Zealand

* To whom correspondence should be addressed. E-mail: peter.hurst{at}stonebow.otago.ac.nz.


  Abstract

Basic fibroblast growth factor (bFGF) is involved in cell proliferation, differentiation, and angiogenesis. It has long been known that bFGF acts as a powerful mitogen for various mammalian granulosa cells in culture. To investigate the possible involvement of bFGF expression in follicle initiation and growth in vivo, we performed nested RT-PCR on ovarian cortical biopsies and quantitative PCR on human follicle populations isolated by laser capture microdissection. Using morphological criteria, follicles were characterized as putative non-growing, primary, or small secondary. RNA was extracted from samples, reverse-transcribed, and relative gene expression levels determined with TaqManTM real-time PCR, using 18S rRNA as the endogenous control. Results confirmed bFGF expression in human adult ovarian cortex, and in the isolated follicles a down-regulation of bFGF mRNA was evident as small follicles develop. This study demonstrates a possible relationship between bFGF mRNA expression and follicle development.

Keywords: bFGF; follicle; laser microdissection; ovary; real-time.
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