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Mol. Hum. Reprod. Advance Access published online on September 10, 2004
Molecular Human Reproduction, doi:10.1093/molehr/gah103
© 2004 by Oxford University Press
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Received July 19, 2004
Accepted August 16, 2004

Article

Labour is associated with increased expression of type-IIA secretory phospholipase A2 but not type-IV cytosolic phospholipase A2 in human myometrium

Donna M. Slater 1*, Shirley Astle 1, Phillip R. Bennett 2, and Steven Thornton 3

1 Biomedical Research Institute, Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
2 Parturition Research Group, Institute of Developmental and Reproductive Biology, Imperial College School of Medicine, London W12 0NN, UK
3 Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK

* To whom correspondence should be addressed. E-mail: D.M.Slater{at}warwick.ac.uk.


  Abstract

Human labour is associated with increased prostaglandin synthesis within the uterus. The aim of this study was to examine the expression of the type-IV cytosolic phospholipase A2 (cPLA2-IV) and the type IIA secretory phospholipase A2 (sPLA2-IIA) in myometrium in association with labour onset at term and preterm deliveries. These enzymes are important for the release of the prostaglandin precursor, arachidonic acid, from phospholipid membrane stores. RT-PCR was used to determine differences in gene expression between non-labour and labour groups. Expression of sPLA2-IIA in human myometrium was significantly increased with pregnancy, and with labour, both at term and preterm. Expression of cPLA2-IV in myometrium was not significantly altered with respect to pregnancy or labour. Immunohistochemical analysis demonstrated differences in the spatial localization of cPLA2-IV and sPLA2-IIA protein in upper and lower segment myometrium. cPLA2-IV was predominantly in vascular endothelial cells, while sPLA2-IIA was observed in vascular, endothelial and smooth muscle cells. In addition, sPLA2-IIA showed a distinct nuclear or perinuclear localization in myometrial smooth muscle cells of the lower segment. We postulate that the increased expression of sPLA2-IIA rather than cPLA2-IV in the myometrium may play a role in the onset and/or maintenance of human parturition.

Keywords: cPLA2; labour; myometrium; preterm; sPLA2.
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