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Mol. Hum. Reprod. Advance Access published online on October 15, 2004
Molecular Human Reproduction, doi:10.1093/molehr/gah112
© 2004 by Oxford University Press
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Received May 6, 2004
Revised August 31, 2004
Accepted September 8, 2004

Article

Mechanical stretch of human uterine smooth muscle cells increases IL-8 mRNA expression and peptide synthesis

J.A.Z. Loudon 1, S.R. Sooranna 2, P.R. Bennett 1, and M.R. Johnson 2*

1 Imperial College Parturition Research Group, Institute of Reproductive and Developmental Biology, Hammersmith Hospital Campus DuCane Road, London W12 0NN, UK
2 Imperial College Parturition Research Group, Department of Maternal Fetal Medicine, Chelsea and Westminster Hospital, 369 Fulham Road, London SW10 9NH, UK

* To whom correspondence should be addressed. E-mail: mark.johnson{at}imperial.ac.uk.


  Abstract

Labour is associated with increased synthesis of interleukin-8 (IL-8) by the fetal membranes and myometrium, which leads to an inflammatory infiltrate. Stretch has been shown to increase the expression of contraction-associated proteins in animal models of labour and in human myocytes in vitro. In this study, we tested the hypothesis that mechanical stretch of human myometrial cells increases IL-8 messenger ribonucleic acid (mRNA) expression. We isolated myocytes from non-pregnant women undergoing hysterectomy and pregnant women undergoing Caesarean section before and after the onset of labour. Myocytes in culture were subjected to stretch of varying intensity (6-16%) and duration (1 or 6 h) using the Flexercell system. IL-8 mRNA expression was lowest in myocytes from pregnant women not in labour, intermediate in those from non-pregnant women and greatest in those from pregnant women in labour. Stretch increased IL-8 mRNA expression independent of reproductive state. The stretch-induced increase in IL-8 mRNA expression was associated with higher IL-8 levels in the culture supernatant and enhanced promoter activity. These data suggest that stretch contributes to the increase in myometrial IL-8 synthesis associated with the onset of labour in humans.

Keywords: interleukin-8; stretch; labour.
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