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Mol. Hum. Reprod. Advance Access published online on October 1, 2004
Molecular Human Reproduction, doi:10.1093/molehr/gah115
© 2004 by Oxford University Press
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Received August 3, 2004
Revised September 9, 2004
Accepted September 12, 2004

Article

Endometrial leptin and leptin receptor expression in women with severe/moderate endometriosis

I. Lima-Couy 1 *, A. Cervero 2 *, F. Bonilla-Musoles 3, A. Pellicer 2, and C. Simón 2*

1 Department of Obstetrics and Gynecology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil; Fundación IVI, School of Medicine, University of Valencia, Valencia, Spain
2 Fundación IVI, School of Medicine, University of Valencia, Valencia, Spain; Department of Pediatrics, Obstetrics and Gynecology, School of Medicine, University of Valencia, Valencia, Spain
3 Department of Pediatrics, Obstetrics and Gynecology, School of Medicine, University of Valencia, Valencia, Spain

* To whom correspondence should be addressed. E-mail: csimon{at}interbook.net.


  Abstract

The leptin system has been implicated in reproductive function, acting at endocrine and paracrine levels. Recently, deregulation of this gene family has been linked to endometrial changes caused by endometriosis. In the present study, we compare the expression of leptin receptor mRNA during the pre-receptive (LH+2) and receptive (LH+9) phases in the eutopic endometrium from patients with severe/moderate endometriosis (n = 30) versus fertile controls (n = 12). In each patient, two endometrial samples were obtained at LH+2 and LH+9 in their natural cycles. When real-time quantitative fluorescent PCR was performed, an up-regulation of OB-RL and all the isoforms investigated was observed at LH+9 versus LH+2 in patients with and without endometriosis. However, no difference was found in the expression pattern of the total leptin receptor OB-RT, or in its long OB-RL and soluble HuB219.3 forms when the eutopic endometria of patients with severe/moderate endometriosis and fertile controls were compared. By means of in situ hybridization, total leptin receptor mRNA was localized in the luminal epithelium and the glands of the endometrium. The immunohistochemical analysis of the long form of leptin receptor was also performed in order to confirm these findings at the protein level. Finally, we have also shown similar leptin mRNA expression in both the control group and patients with endometriosis. In conclusion, we have not identified differences in the endometrial expression and localization of leptin and the leptin receptor when comparing the eutopic endometrium of women with severe/moderate endometriosis and fertile controls.

Keywords: endometriosis; implantation; leptin; leptin receptor.

* I.L. and A.C. contributed equally.


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