Skip Navigation



Mol. Hum. Reprod. Advance Access published online on December 10, 2004
Molecular Human Reproduction, doi:10.1093/molehr/gah137
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
11/2/133    most recent
gah137v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Dadoune, J.P.
Right arrow Articles by Siffroi, J.P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dadoune, J.P.
Right arrow Articles by Siffroi, J.P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction © European Society of Human Reproduction and Embryology 2004; all rights reserved
Received September 28, 2004
Revised November 10, 2004
Accepted November 17, 2004

Article

Identification of transcripts by macroarrays, RT-PCR and in situ hybridization in human ejaculate spermatozoa

J.P. Dadoune 1*, Pawlak A. 2, M.F. Alfonsi 3, and J.P. Siffroi 4

1 Laboratoire de Cytologie et Histologie, Centre Universitaire des Saints-Pères, 75270, Paris, France, EA 1533 Génétique de la Reproduction Humaine, Hôpital Tenon (AP-HP), 75970, Paris, France
2 Unité INSERM 581, Hôpital Henri-Mondor, 94010 Créteil, Paris, France
3 Laboratoire de Cytologie et Histologie, Centre Universitaire des Saints-Pères, 75270, Paris, France
4 Laboratoire de Cytologie et Histologie, Centre Universitaire des Saints-Pères, 75270, Paris, France, EA 1533 Génétique de la Reproduction Humaine, Hôpital Tenon (AP-HP), 75970, Paris, France

* To whom correspondence should be addressed.
J.P. Dadoune, E-mail: jean-pierre.dadoune{at}univ-paris5.fr


  Abstract

Round spermatids contain high levels of extremely varied mRNAs that are synthesized either throughout early spermatogenesis or during spermiogenesis from the haploid genome. Concomitantly, with major changes in the chromatin organization, arrest of transcription occurs at midspermiogenesis. However, previous investigations using RT-PCR have revealed the persistence of numerous and different transcripts in ejaculated spermatozoa. In the present study, a step-by-step analysis by means of macroarray hybridization, RT-PCR and in situ hybridization was performed to identify more accurately the different mRNA species found in the human ejaculated spermatozoa. The data showed an extended pattern of various transcripts encoding a diverse range of proteins involved in signal transduction and cell proliferation. For the first time, they demonstrated that mRNAs coding for the transcription factors NF{kappa}B, HOX2A, ICSBP, protein kinase JNK2, growth factor HBEGF and receptors RXR{beta} and ErbB3 accumulate within the sperm nucleus. The origin and fate of the sperm transcripts remain subject to discussion.

Keywords: haploid genome; messenger RNAs; spermatozoa.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ReproductionHome page
N. Bissonnette, J.-P. Levesque-Sergerie, C. Thibault, and G. Boissonneault
Spermatozoal transcriptome profiling for bull sperm motility: a potential tool to evaluate semen quality
Reproduction, July 1, 2009; 138(1): 65 - 80.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. Avendano, A. Franchi, E. Jones, and S. Oehninger
Pregnancy-specific {beta}-1-glycoprotein 1 and human leukocyte antigen-E mRNA in human sperm: differential expression in fertile and infertile men and evidence of a possible functional role during early development
Hum. Reprod., February 1, 2009; 24(2): 270 - 277.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. Lalancette, C. Thibault, I. Bachand, N. Caron, and N. Bissonnette
Transcriptome Analysis of Bull Semen with Extreme Nonreturn Rate: Use of Suppression-Subtractive Hybridization to Identify Functional Markers for Fertility
Biol Reprod, April 1, 2008; 78(4): 618 - 635.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
I. Gilbert, N. Bissonnette, G. Boissonneault, M. Vallee, and C. Robert
A molecular analysis of the population of mRNA in bovine spermatozoa
Reproduction, June 1, 2007; 133(6): 1073 - 1086.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
H.-G. Li, X.-F. Ding, A.-H. Liao, X.-B. Kong, and C.-L. Xiong
Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility
Mol. Hum. Reprod., May 1, 2007; 13(5): 299 - 306.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
Z.-M. Cai, Y.-T. Gui, X. Guo, J. Yu, L.-D. Guo, L.-B. Zhang, H. Wang, and J. Yu
Low Expression of Glycoprotein Subunit 130 in Ejaculated Spermatozoa from Asthenozoospermic Men
J Androl, September 1, 2006; 27(5): 645 - 652.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
R. K. Naz and R. Sellamuthu
Receptors in Spermatozoa: Are They Real?
J Androl, September 1, 2006; 27(5): 627 - 636.
[Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. Schuel and L. J. Burkman
A Tale of Two Cells: Endocannabinoid-Signaling Regulates Functions of Neurons and Sperm
Biol Reprod, December 1, 2005; 73(6): 1078 - 1086.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
D Modi, C Shah, G Sachdeva, S Gadkar, D Bhartiya, and C Puri
Ontogeny and cellular localization of SRY transcripts in the human testes and its detection in spermatozoa
Reproduction, November 1, 2005; 130(5): 603 - 613.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.