Skip Navigation



Mol. Hum. Reprod. Advance Access published online on February 25, 2005
Molecular Human Reproduction, doi:10.1093/molehr/gah159
This Article
Right arrow Advance Access manuscript (PDF) Freely available
Right arrow All Versions of this Article:
11/4/253    most recent
gah159v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Geva, E.
Right arrow Articles by Jaffe, R.B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Geva, E.
Right arrow Articles by Jaffe, R.B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction © European Society of Human Reproduction and Embryology 2005; all rights reserved
Received October 14, 2004
Accepted January 21, 2005

Article

In utero angiopoietin-2 gene delivery remodels placental blood vessel phenotype: a murine model for studying placental angiogenesis

E. Geva 1, D.G. Ginzinger 2, D.H. Moore II2, P.C. Ursell 3, and R.B. Jaffe 1*

1 Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, USA
2 UCSF Comprehensive Cancer Center, University of California, USA
3 Department of Pathology, University of California, San Francisco, CA, USA

* To whom correspondence should be addressed.
R.B. Jaffe, E-mail: jaffer{at}obgyn.ucsf.edu


  Abstract

Angiopoietin (Ang)-2, the natural antagonist of the Ang1/Tie2 receptor is a complex regulator of blood vessel plasticity that plays a pivotal role in both vessel sprouting [in the presence of vascular endothelial growth factor (VEGF)-A] and vessel regression (in the absence of VEGF-A). Based on the spatial and temporal expression of Ang2 throughout human gestation, we recently suggested that the Ang2 may play a pivotal role in placental angiogenesis. Further, to examine this tenet we have developed a novel murine model system in which in utero Ang2 gene delivery via a non-replicating adenoviral expression vector has the potential to manipulate the blood vessel phenotype in vivo during pregnancy. Ang2 overexpression selectively and rapidly remodels the labyrinth perivascular extracellular matrix, subsequently promoting plasticity of the maternal and fetal vessels, which appear honeycombed due to a 2-fold increase in blood vessel luminal area. High levels of Ang2 impair endothelial cell adhesiveness, leading to vascular leakiness with perivascular oedema, which increases placental weight. These observations suggest that the Ang2 overexpression may play a key role in placental vascular remodelling. Furthermore, we suggest a novel new model to study the pathobiology of placental vascularization and the effect of placental blood vessels on fetal phenotype.

Keywords: angiogenesis; angiopoietin-2; placenta.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
I.S. Glinert, E. Geva, C. Tempel-Brami, A. Brandis, A. Scherz, and Y. Salomon
Photodynamic ablation of a selected rat embryo: a model for the treatment of extrauterine pregnancy
Hum. Reprod., July 1, 2008; 23(7): 1491 - 1498.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
H. Ishimoto, K. Minegishi, T. Higuchi, M. Furuya, S. Asai, S. H. Kim, M. Tanaka, Y. Yoshimura, and R. B. Jaffe
The Periphery of the Human Fetal Adrenal Gland Is a Site of Angiogenesis: Zonal Differential Expression and Regulation of Angiogenic Factors
J. Clin. Endocrinol. Metab., June 1, 2008; 93(6): 2402 - 2408.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
B. Davis, A. Dei Cas, D. A. Long, K. E. White, A. Hayward, C.-H. Ku, A. S. Woolf, R. Bilous, G. Viberti, and L. Gnudi
Podocyte-Specific Expression of Angiopoietin-2 Causes Proteinuria and Apoptosis of Glomerular Endothelia
J. Am. Soc. Nephrol., August 1, 2007; 18(8): 2320 - 2329.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.