Mol. Hum. Reprod. Advance Access published online on March 11, 2005
Molecular Human Reproduction, doi:10.1093/molehr/gah161
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1 Laboratory for Research in Reproductive Sciences, Department of Obstetrics and Gynecology,, Ha'Emek Medical Center, Afula, Israel
* To whom correspondence should be addressed. In humans, progesterone levels are sustained before the onset of labour. Therefore, the mechanism for parturition that has been proposed for humans is ‘functional’ progesterone withdrawal. Immunohistochemical staining for the progesterone receptor (PR) was positive in the decidua with a decline after contractions began. Western blot analysis revealed a number of PR isoforms expressed in the decidua, with the PR-B form being dominant. After contractions began, all PR isoforms decreased sharply. PR-B and PR-A decreased by 85.8%±6.7 and 78.2%±7.1, respectively (P<0.001). Incubation of decidua with Prostaglandin F2
Received January 8, 2005
Accepted February 2, 2005
Article
Progesterone receptor expression in human decidua and fetal membranes before and after contractions: possible mechanism for functional progesterone withdrawal
2 Pathology Department, Ha'Emek Medical Center, Afula, Israel
3 Laboratory for Research in Reproductive Sciences, Department of Obstetrics and Gynecology,, Ha'Emek Medical Center, Afula, Israel Rappaport Faculty of Medicine, Technion-Institute of Technology, Haifa, Israel
Eliezer Shalev, E-mail: shaleve{at}tx.technion.ac.il
Abstract 
1.0 µg/ml decreased the expression of all forms of PR isoforms. PR-B was reduced by 64%±6.09 (P<0.01); PR-A was reduced by 77%±5.9 (P<0.05), while PR-C was reduced by 80%±7.24 (P<0.05). Progesterone (80 µg/ml) increased the PR-B, PR-C the 45 and 36 kDa isoforms to 150%±7.89, 210%±12.4, 270%±9.7 and 216%±13.5, respectively (P<0.05). In immunohistochemical studies, the PR was not identified in the amnion or in the chorion, regardless of the presence or absence of contractions. Western blot analysis demonstrated that PR-C (60 kDa) and the 36 kDa isoforms were dominant in the amnion. After contractions began, PR-A decreased significantly by 61.9%±7.1 (P<0.001).
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