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Mol. Hum. Reprod. Advance Access first published online on August 26, 2005
This version published online on September 13, 2005
Molecular Human Reproduction, doi:10.1093/molehr/gah203
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received May 12, 2005
Revised May 27, 2005
Accepted June 12, 2005

Article

Expression and regulation of Sprouty-2 in the granulosa-lutein cells of the corpus luteum

Ronit Haimov-Kochman 1, Amir Ravhon 1, Diana Prus 2, Caryn Greenfield 1, Zvesdana Finci-Yeheskel 1, Debra S.Goldman-Wohl 1, Shira Natanson-Yaron 1, Reuven Reich 3, Simcha Yagel 1, and Arye Hurwitz 1*

1 Department of Obstetrics and Gynecology, Hadassah University Medical Center, Jerusalem, Israel
2 Department of Pathology, Hadassah University Medical Center, Jerusalem, Israel
3 Department of Clinical Pharmacology, The Hebrew University, Jerusalem, Israel

* To whom correspondence should be addressed.
Arye Hurwitz, E-mail: hurwitz{at}cc.huji.ac.il


  Abstract

Growth factor signalling has important modulatory roles in the process of human follicular growth, oocyte maturation and corpus luteum (CL) formation. Recently, Sprouty-2, an inhibitor of receptor tyrosine kinase (RTK) signalling pathway was advocated as a marker of oocyte competence in the bovine ovary. We sought to study Sprouty-2 expression and regulation in the human ovary. RT-PCR was used to detect Sprouty-2 mRNA in human granulosa-lutein cells (GLC) collected from follicular aspiration of IVF patients. The effect of epidermal and fibroblast growth factors (EGF and FGF) on Sprouty-2 mRNA expression in GLC was studied using quantitative real-time PCR. Immunohistochemistry was performed on cultured GLC, human CL and stimulated rat ovary sections. Sprouty-2 mRNA was expressed in human GLC. EGF and basic FGF, but not FGF4 and FGF10, increased Sprouty-2 mRNA expression in GLC. The Sprouty protein was localized to GLC of early and late human CL but not to the theca cell layer. Immunostaining of developing rat CL confirmed the temporal and spatial expression of Sprouty in humans. The detection of Sprouty-2 mRNA and protein in human GLC may suggest a role for Sprouty-2 during the final stages of follicle maturation and CL formation.

Keywords: corpus luteum/follicle/granulosa/ovary/Sprouty.
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