Mol. Hum. Reprod. Advance Access published online on March 23, 2006
Molecular Human Reproduction, doi:10.1093/molehr/gal011
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1 Department of Obstetrics and Gynecology, Lund University Hospital, Lund, Sweden
* To whom correspondence should be addressed. The aim of this study was to investigate patterns of gene expression in placental samples from patients with preeclampsia (PE), persistent bilateral uterine artery notching (without PE), and normal controls. This study included placental tissue from nine women with PE, seven with uncomplicated pregnancies and five with bilateral uterine artery notching in Doppler velocimetry tracings. Human cDNA microarrays with 6500 transcripts/genes were used and the results verified with real-time PCR and in-situ hybridization. Multidimensional scaling method and random permutation technique demonstrated significant differences among the three groups examined. Within the 6.5K arrays, 6198 elements were unique cDNA clones representing 5952 unique UniGenes and 5695 unique LocusLinks. Multidimensional scaling plots showed 5000 genes that met our quality criteria; among these, 366 genes were significantly different in at least one comparison. Differences in three genes of interest were confirmed with real-time PCR and in-situ hybridization; acid phosphatase 5 was shown to be overexpressed in PE samples and calmodulin 2 and v-rel retic-uloendotheliosis viral oncogene homolog A (RELA) were downregulated in PE and uterine artery notch placentas. In conclusion downregulation of RELA and calmodulin 2 might represent an attempt by the placenta to compensate for elevations in intracellular calcium, possibly caused by hypoxia and/or apoptosis, in both pregnancies with uterine artery notching and preeclampsia.
Received December 1, 2005
Revised December 28, 2005
Accepted January 9, 2006
Article
Gene expression profiling of human placentas from preeclamptic and normotensive pregnancies
S.R. Hansson 1 *,
Y. Chen 2,
J. Brodszki 1,
M. Chen 3,
E. Hernandez-Andrade 1,
J.M. Inman 4,
O.A. Kozhich 4,
I. Larsson 1,
K. Marsál 1,
P. Medstrand 5,
C.C. Xiang 3,
and
M.J. Brownstein 3
2 Cancer Genetics Branch, National Human Genome Research Institute
3 Laboratory of Genetics, National Institutes of Mental Health, National Institutes of Health, Bethesda
4 Laboratory of Genetics, National Institutes of Mental Health, National Institutes of Health, Bethesda; SAIC Frederick, National Cancer Institute at Frederick, Fredrick, MD, USA
5 Department of Cell and Molecular Biology, Lund University, Lund, Sweden
S.R. Hansson, E-mail: stefan.hansson{at}med.lu.se
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