Mol. Hum. Reprod. Advance Access published online on April 28, 2006
Molecular Human Reproduction, doi:10.1093/molehr/gal033
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1 Infertility and IVF Unit; Helen Schneider’s Women Hospital, Rabin Medical Center, Petach Tikva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
* To whom correspondence should be addressed. The signals initiating the growth of primordial follicles are unknown. Growth factors such as neurotrophin 4/5 (NT-4/5) and brain-derived neurotrophic factor (BDNF) may play a role in this process. To investigate the expression of NT-4/5 and BDNF and their receptor tyrosine kinase B (TrkB) in the early developing follicles, we fixed and froze 12 ovarian samples from adolescents/ adults and 31 ovaries from human fetuses. The fixed samples were prepared for immunohistochemical staining for NT-4/5, BDNF and the TrkB receptor. Total RNA was extracted from the frozen ovarian samples, and the expression of NT-4/5, BDNF and the TrkB receptor (full length and two truncated isoforms) was investigated by RT-PCR. Products were resolved by 1% agarose gel electrophoresis and image analysis. Immunohistochemical staining revealed the expression of NT-4/5 and BDNF mainly in oocytes and, in a minority of samples, also in the granulosa cells (GCs); TrkB receptor was identified in oocytes and GCs. Transcripts of NT-4/5, BDNF and all forms of TrkB receptor were identified in the samples. To elucidate whether indeed NT-4/5 and BDNF are involved in growth initiation of human primordial follicles, they should be added to the culture medium.
Received January 11, 2006
Accepted February 28, 2006
Article
Tyrosine kinase B receptor and its activated neurotrophins in ovaries from human fetuses and adults
S. Harel 1,
S. Jin 2,
B. Fisch 3,
D. Feldberg 4,
H. Krissi 4,
C. Felz 5,
S. Freimann 6,
S.L. Tan 2,
A. Ao 7,
and
R. Abir 1 *
2 Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada
3 Infertility and IVF Unit
4 Helen Schneider’s Women Hospital, Rabin Medical Center, Petach Tikva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
5 Department of Pathology, Rabin Medical Center
6 The Felsenstein Medical Research Center, Petach Tikva and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
7 Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada; Department of Human Genetics, McGill University, Montreal, Quebec, Canada
R. Abir, E-mail: ronita{at}clalit.org.il
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