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Mol. Hum. Reprod. Advance Access published online on August 25, 2006
Molecular Human Reproduction, doi:10.1093/molehr/gal061
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received March 21, 2006
Revised June 1, 2006
Accepted June 7, 2006

Article

Myometrial prostaglandin E2 synthetic enzyme mRNA expression: spatial and temporal variations with pregnancy and labour

S.R. Sooranna 1, P.L. Grigsby 2, N. Engineer 1, Z. Liang 3, K. Sun 2, L. Myatt 2, and M.R. Johnson 1 *

1 Imperial College Parturition Research Group, Department of Maternal Fetal Medicine, Imperial College School of Medicine, Chelsea and Westminster Hospital, London, UK
2 Department of Obstetrics & Gynecology, University of Cincinnati College of Medicine, Cincinnati, OH, USA
3 Imperial College Parturition Research Group, Department of Maternal Fetal Medicine, Imperial College School of Medicine, Chelsea and Westminster Hospital, London, UK; Department of Obstetrics & Gynecology, Southwest Hospital, Third Military Medical University, Chongqing, China

* To whom correspondence should be addressed.
M.R. Johnson, E-mail: mark.johnson{at}imperial.ac.uk


  Abstract

We have investigated the hypothesis that the expression of the enzymes involved in PGE2 synthesis in the human uterus is coordinated. We have studied (i) the mRNA expression of the enzymes involved in PGE2 synthesis [phospholipases (cPLA2 and sPLA2), prostaglandin H synthase (PGHS)-2 and PG E synthases (PGES-1 and -2)] and their relationship to the expression of inflammatory cytokines in samples of myometrium obtained from pregnant women undergoing caesarean section (LSCS) either before or after the onset of labour at or before term; and (ii) the effect of IL-1{beta}, IL-6, TNF-{alpha}, PGE2 and stretch on PGE2 enzyme mRNA expression. We found that cPLA2, sPLA2 and PGHS-2 mRNA expression were greater in labour samples; cPLA2, sPLA2, PGHS-2, PGES-1 and -2 mRNA expression were greater in lower- than upper-segment samples; and there was no effect of gestational age. PGHS-2 mRNA levels correlated with those of PGES-1, cPLA2, IL-1{beta} and IL-8; PGES-1 mRNA levels correlated with those of IL-1{beta}, IL-8 and cPLA2. In primary cultures of uterine myocytes, cPLA2 mRNA expression was increased by IL-1{beta} and IL-6; PGHS-2 mRNA expression was increased by IL-1{beta}, PGE2 and stretch; and PGES-1 mRNA expression was increased by IL-1{beta} only. These data show that labour is associated with increased expression of the enzymes involved in PGE2 synthesis and their expression is greater in the lower uterine segment. The presence of associations between the levels of PGE2 enzyme mRNA expression and the effects of IL-1{beta} suggest that their expression is co-ordinated and that IL-1{beta} is the responsible factor.

Keywords: cytokines/labour/prostaglandin synthesis/stretch/uterine smooth muscle cells.
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