Mol. Hum. Reprod. Advance Access published online on August 31, 2007
Molecular Human Reproduction, doi:10.1093/molehr/gam063
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The Expression Profile of Micro-RNA In Endometrium and Endometriosis and the Influence of Ovarian Steroids on their Expression
Department of OB/GYN University of Florida, Gainesville, Fl 32610
1 Address Correspondence: Dr. Nasser Chegini Department of OB/GYN, University of Florida, Box 100294, Gainesville FL. 32610, Tel: (352) 392-3929, Fax: (352) 392-6994, E.Mail: cheginin{at}obgyn.ufl.edu
Abstract: MicroRNAs (miRNAs), through mRNA degradation or repression, act as key regulator of gene expression. Our aim was to identify specific miRNAs that are expressed in endometrium of women with and without endometriosis. We profiled the expression of 287 miRNAs in paired eutopic and ectopic endometrium and isolated endometrial cells using microarray and validated the expression of selected miRNAs using realtime PCR. Based on global normalization, 65 of these miRNAs were identified to be expressed above the threshold levels set during the analysis in the endometrium of women without endometriosis with a progressive decline in expression in paired eutopic and ectopic endometrium. Statistical analysis (ANOVA) identified 48 of these miRNAs as differentially expressed among these tissues and 32 miRNAs between isolated endometrial stromal (ESC) and glandular epithelial (GEC) cells (P<0.05). The expression of hsa-miR20a, hsa-miR21, hsa-miR26a, hsa-miR18a, hsa-miR206, hsa-miR181a, and hsa-miR142-5p, predicated to target many genes, including TGF-ßR2, ER
, ERß and PR, respectively was validated in these tissues and cells using realtime PCR. Treatment of ESC and GEC with 17ß estradiol and medroxyprogesterone acetate (10–8 M) differentially regulated the expression of hsa-miR20a, hsa-miR21, hsa-miR26a, which in part reversed following co-treatment with ICI-182780 and RU-486 (10–6 M), respectively (P<0.05). In conclusion, we provided evidence for the expression of a number of differentially expressed miRNAs in eutopic/ectopic endometrium and isolated endometrial cells, opening up the possibility that aberrant/altered expression of some miRNAs whose expression is regulated by the ovarian steroids may influence the expression of specific target genes with central roles in normal endometrial cellular activities and pathogenesis of endometriosis.
Key Words: endometrium/endometriosis/miRNA/expression/regulation
Submitted on July 27, 2007; accepted on August 21, 2007.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Maillot, M. Lacroix-Triki, S. Pierredon, L. Gratadou, S. Schmidt, V. Benes, H. Roche, F. Dalenc, D. Auboeuf, S. Millevoi, et al. Widespread Estrogen-Dependent Repression of microRNAs Involved in Breast Tumor Cell Growth Cancer Res., November 1, 2009; 69(21): 8332 - 8340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Qian, L. Hu, H. Chen, H. Li, N. Liu, Y. Li, J. Ai, G. Zhu, Z. Tang, and H. Zhang Hsa-miR-222 Is Involved in Differentiation of Endometrial Stromal Cells in Vitro Endocrinology, October 1, 2009; 150(10): 4734 - 4743. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.G. Braundmeier and A.T. Fazleabas The non-human primate model of endometriosis: research and implications for fecundity Mol. Hum. Reprod., October 1, 2009; 15(10): 577 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-W. Guo Epigenetics of endometriosis Mol. Hum. Reprod., October 1, 2009; 15(10): 587 - 607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.O. Burney, A.E. Hamilton, L. Aghajanova, K.C. Vo, C.N. Nezhat, B.A. Lessey, and L.C. Giudice MicroRNA expression profiling of eutopic secretory endometrium in women with versus without endometriosis Mol. Hum. Reprod., October 1, 2009; 15(10): 625 - 631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. C. Ohlsson Teague, C. G. Print, and M. L. Hull The role of microRNAs in endometriosis and associated reproductive conditions Hum. Reprod. Update, September 22, 2009; (2009) dmp034v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. S. Wickramasinghe, T. T. Manavalan, S. M. Dougherty, K. A. Riggs, Y. Li, and C. M. Klinge Estradiol downregulates miR-21 expression and increases miR-21 target gene expression in MCF-7 breast cancer cells Nucleic Acids Res., May 1, 2009; 37(8): 2584 - 2595. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Z. Carletti and L. K. Christenson MicroRNA in the ovary and female reproductive tract J Anim Sci, April 1, 2009; 87(14_suppl): E29 - E38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. C. Ohlsson Teague, K. H. Van der Hoek, M. B. Van der Hoek, N. Perry, P. Wagaarachchi, S. A. Robertson, C. G. Print, and L. M. Hull MicroRNA-Regulated Pathways Associated with Endometriosis Mol. Endocrinol., February 1, 2009; 23(2): 265 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Toloubeydokhti, Qun Pan, Xiaoping Luo, O. Bukulmez, and N. Chegini The Expression and Ovarian Steroid Regulation of Endometrial Micro-RNAs Reproductive Sciences, December 1, 2008; 15(10): 993 - 1001. [Abstract] [PDF]
|
|
|
|
|
|
X. Hong, L. J. Luense, L. K. McGinnis, W. B. Nothnick, and L. K. Christenson Dicer1 Is Essential for Female Fertility and Normal Development of the Female Reproductive System Endocrinology, December 1, 2008; 149(12): 6207 - 6212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Hull, C. R. Escareno, J. M. Godsland, J. R. Doig, C. M. Johnson, S. C. Phillips, S. K. Smith, S. Tavare, C. G. Print, and D. S. Charnock-Jones Endometrial-Peritoneal Interactions during Endometriotic Lesion Establishment Am. J. Pathol., September 1, 2008; 173(3): 700 - 715. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-J. Hu, G. Ren, J.-L. Liu, Z.-A. Zhao, Y.-S. Yu, R.-W. Su, X.-H. Ma, H. Ni, W. Lei, and Z.-M. Yang MicroRNA Expression and Regulation in Mouse Uterus during Embryo Implantation J. Biol. Chem., August 22, 2008; 283(34): 23473 - 23484. [Abstract] [Full Text] [PDF]
|
|