Skip Navigation



Mol. Hum. Reprod. Advance Access published online on October 31, 2007
Molecular Human Reproduction, doi:10.1093/molehr/gam075
This Article
Right arrow Advance Access manuscript (PDF)
Right arrow All Versions of this Article:
13/12/845    most recent
gam075v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Mönkkönen, K. S.
Right arrow Articles by Fazeli, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mönkkönen, K. S.
Right arrow Articles by Fazeli, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Hormonal regulation of G{alpha}i2 and mPR{alpha} in immortalized human oviductal cell line OE-E6/E7

Kati S. Mönkkönen1,2, Reza Aflatoonian2, Kai-Fai Lee3, William S.B. Yeung3, Sai-Wah Tsao4, Jarmo T. Laitinen5 and Alireza Fazeli2,

1Department of Pharmacology & Toxicology, University of Kuopio, Kuopio, FIN-70211, Finland 2Academic Unit of Reproductive and Developmental Medicine, The University of Sheffield, Level 4, Jessop Wing, Tree Root Walk, Sheffield S10 2SF, UK 3Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong 4Department of Anatomy, The University of Hong Kong, Pokfulam, Hong Kong 5Institute of Biomedicine, Department of Physiology, University of Kuopio, Kuopio, FIN-70211, Finland

Corresponding author: Alireza Fazeli, Academic Unit of Reproductive and Developmental Medicine, The University of Sheffield, Level 4, The Jessop Wing, Sheffield S10 2SF, UK. Tel. +44 114 226 8195, Fax +44 114 226 1074, E-mail: a.fazeli{at}sheffield.ac.uk

Heterotrimeric G proteins play a key role in membrane-mediated cell-signalling and hormonal regulation. Our earlier studies gave evidence of G protein subunit G{alpha}i2 being under hormonal regulation in human in vivo. In this study we used immortalised human oviduct epithelial cell line OE-E6/E7 as a model to study the hormonal regulation of G{alpha}i2. We aimed at clarifying whether estradiol or progesterone could individually regulate the expression of G{alpha}i2 and its potential signalling partners. Furthermore, we aimed to investigate which sex hormone receptors could potentially mediate the gene regulation in OE-E6/E7 cell line. OE-E6/E7 cells were cultured for 5 days with different concentrations of estradiol or progesterone. Quantitative real-time polymerase chain reaction (Q-PCR) was performed using cDNA of the hormone treated cells to reveal any changes in gene expression. The presence of potential receptor targets in these cells was studied using PCR. Our data clearly showed that low concentrations of estradiol up-regulated the expression of G{alpha}i2 gene and down-regulated the expression of membrane progesterone receptor mPR{alpha} gene in OE-E6/E7 cell line. Progesterone had no significant effect on G{alpha}i2 gene expression but it caused up-regulation of mPR{alpha} gene expression.: In conclusion, it appears that sex hormones regulate the expression of G{alpha}i2 and mPR{alpha} genes in a reverse manner in OE-E6/E7 cells. Our results suggest estrogen receptor ERß mediates the regulatory effects of estradiol in these cells.

Submitted on September 2, 2007; accepted on September 24, 2007.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.