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Mol. Hum. Reprod. Advance Access published online on February 4, 2008
Molecular Human Reproduction, doi:10.1093/molehr/gan005
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Germline Expression of Mammalian CTF18, an Evolutionarily Conserved Protein Required for Germ Cell Proliferation in the Fly and Sister Chromatid Cohesion in Yeast

Karen M. Berkowitz1,3,, Klaus H. Kaestner2 and Thomas A. Jongens1,2

1Center for Research on Reproduction and Women's Health, University of Pennsylvania, Philadelphia, PA 19104 2Department of Genetics, University of Pennsylvania, Philadelphia, PA 19104 3Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, PA 19104

Correspondence: Karen M. Berkowitz, Center for Research on Reproduction and Women's Health, School of Medicine, University of Pennsylvania, 1307 BRB II/III, 421 Curie Blvd., Philadelphia, PA 19104-6160. Telephone: 215-573-1944, Fax: 215-573-7627, Email: kberkowitz{at}obgyn.upenn.edu

Cutlet/CTF18 encodes an evolutionarily conserved protein that is crucial for germline development in Drosophila melanogaster. Loss of function of cutlet in the fly results in a sterile phenotype due to the failure of germline stem cells to proliferate. CTF18 was first identified in Saccharomyces cerevisiae as a sister chromatid cohesion factor that is essential for the faithful transmission of chromosomes during DNA replication. We have cloned and characterized the human and mouse CTF18 orthologues of the Drosophila melanogaster gene, cutlet. We have demonstrated that CTF18 mRNA is expressed in human and mouse testis and ovary, and that CTF18 protein is expressed throughout the male and female germline of the mouse. We suggest a unique biological role for CTF18 in mammalian germ cell development based on its mammalian germline expression, high degree of evolutionary conservation, and role in DNA replication and chromosomal stability in yeast.

Key Words: CTF18/DNA Replication/Germ Cell Development/Germline Expression/CTF18-Replication factor C-like complex (RLC)

Submitted on June 20, 2007; resubmitted on January 17, 2008; accepted on January 22, 2008.


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