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Mol. Hum. Reprod. Advance Access published online on March 7, 2008
Molecular Human Reproduction, doi:10.1093/molehr/gan011
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Platelet-derived growth factors (PDGF-A and B) and their receptors in human fetal and adult ovaries

H. Pinkasa,b, B. Fischa,b, G. Rozanskyc, C. Felza,b, G. Kessler-Iceksone, H. Krissib, S. Nitkeb, A. Aoc,d and R. Abira,b,

a Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Campus, Petach Tikva, 49100 and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel b Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Campus, Petach Tikva, 49100 and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel c Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec H3A 1A1, Canada d Department of Human Genetics, McGill University, Montreal, Quebec H3A 1A1, Canada e Felsenstein Medical Research Center, Beilinson Campus, Petach Tikva 49100 and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

Address for correspondence: Ronit Abir, Ph.D., IVF Research Laboratory, Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Campus, Petach Tikva, 49100, Israel (Fax: 972 3 9240533; e mail: ronita{at}clalit.org.il)

There is no information regarding the presence of platelet-derived growth factors (PDGFs) and their receptors in human ovaries. The expression of PDGF-A, PDGF-B, and their two receptors, PDGFR-{alpha} and PDGFR-β, was investigated in ovarian samples from women/girls and from human fetuses, at the protein and mRNA levels. The samples were prepared for immunohistochemical staining for PDGF-A and PDGF-B and their two receptors and in situ hybridization for the detection of the mRNA transcripts of the receptors. Total RNA was extracted from frozen ovarian samples, and the expression of PDGF-A and PDGF-B was investigated by reverse transcription polymerase chain reaction. The proteins for PDGF-A and PDGF-B were detected in oocytes, and in granulosa cells (GC) of 50% of the follicles from women/girls. The proteins and mRNA transcripts for the two receptors were detected in oocytes (mRNA for PDGFR-β only in 25% of the oocytes). PDGFR-{alpha} mRNA was expressed in GC of a minority of the samples from women/girls, whereas PDGFR-β protein and mRNA were identified in over 50% of the GC from this source. PDGF-A and -B transcripts were identified in all the extracts. The presence of the receptors in GC suggests that PDGFs might be involved in the activation of primordial follicles.

Key Words: primordial follicles/PDGFs and receptors/immunohistochemistry/in situ hybridization/RT-PCR

Submitted on January 8, 2008; resubmitted on February 25, 2008; accepted on February 26, 2008.


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