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Mol. Hum. Reprod. Advance Access published online on May 20, 2008
Molecular Human Reproduction, doi:10.1093/molehr/gan024
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Identification of target messenger RNA substrates for mouse RBMY

Mei Zeng1, Huaqin Sun1, Shu Chen1, Xinying Wang1, Yuan Yang1, Yunqiang Liu1, Dachang Tao1, Zhirong Yang2, Sizhong Zhang1 and Yongxin Ma1,*

1Department of Medical Genetics, West China Hospital; Division of Human Morbid Genomics, State Key Laboratory of Biotherapy, Sichuan university, Chengdu, 610041, P.R.China 2Key laboratory of bio-resources and Eco-environments, ministry of education

* Address for correspondence and reprints request: Prof.Yongxin Ma, Renmin Nanlu, Section 3 #17, Chengdu, 610041, P.R.China. Tel: +86-28-85164009; Fax: +86-28-85164009. E-mail address of the corresponding author: mayongxing{at}263.net or mayongxin{at}gmail.com

Rbmy gene encodes a RNA-binding protein and its expression is limited to the nuclei of germ cells. Previous studies indicate that RBMY may function in pre-mRNA processing during spermatogenesis, although its precise target mRNAs remain unclear. By using specific nucleic acids associated with proteins (SNAAP) and immunoprecipitation techniques, we have identified 12 potential target mRNAs bound by mouse RBMY protein from testis. We detect that both mRbmy-1 and mRbmy-2 transcripts coexist in mouse testis and they differ mainly in the 5’UTR. Importantly, our result shows that mRBMY protein can bind to one of its own transcripts, mRbmy-2, suggesting that mRBMY may affect alternative splicing or regulate the expression of its own gene. Using electrophoretic mobility shift assay we demonstrated that mRBMY protein can bind to the testis and sperm-specific spa17 mRNA and that the binding domain contains rich oligo(A), suggesting that mRBMY protein may have high affinity to oligo(A) rich sequences. In conclusion, the identification of RBMY target mRNAs will be helpful to further explore the biological function of RBMY in spermatogenesis.

Key Words: RBMY/target mRNAs/spermatogenesis/mouse

Submitted on December 18, 2007; resubmitted on April 4, 2008; accepted on May 2, 2008.


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