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Mol. Hum. Reprod. Advance Access published online on May 20, 2008
Molecular Human Reproduction, doi:10.1093/molehr/gan032
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

CXCL10 and IL-6 induce chemotaxis in human trophoblast cell lines

F. Dominguez1, S. Martínez1, A. Quiñonero1, F. Loro1, J.A. Horcajadas1, A. Pellicer1,2 and C. Simón1,2

1Fundación Instituto Valenciano de Infertilidad (FIVI), University of Valencia. C/ Guadassuar 1, bajo Valencia, Spain. 46015 2Dept of Pediatrics, Obstetrics and Gynecology. School of Medicine, University of Valencia, Av. Blasco Ibáñez 17, Valencia, Spain. 46010

Correspondence e-mail: csimon{at}ivi.es Reprints address: Carlos Simon. C/ Guadassuar 1 bajo. Valencia, Spain. 46015

The investigation of trophoblast chemoattractive molecules in humans is of high interest for the reproductive field. Current evidence in ruminants demonstrates that CXCL10, formerly the interferon-{gamma}-inducible protein 10 (IP-10), is a potent chemotactic molecule implicated in the migration of trophoblast cells during early gestation. The aim of this work was to explore the existence of CXCL10/CXCR3 in the human model. Furthermore, chemotaxis assays were performed to demonstrate CXCL10 chemotactic activity in the human trophoblast cell lines JEG-3 and AC-1M88. Surprisingly, the conditioned media from epithelial endometrial cells (EEC) induced the highest trophoblast migration rate. Cytokine and chemokine membrane protein arrays were used to identify the secreted protein profile of EEC-conditioned media, and IL-6 was found to be the most abundant and CXCL13 the second most abundant molecule. Using a chemotaxis assay on AC-IM88, IL-6 antibody blocked the effect of EEC, indicating IL-6 to be an effective chemoattractive factor for trophoblast cells in the human model.

Key Words: CXCL10/chemotaxis/implantation/IL-6

Abbreviated title: CXCL10/IL-6 chemoattracts trophoblast cell lines

Submitted on April 15, 2008; resubmitted on May 13, 2008; accepted on May 15, 2008.


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