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Mol. Hum. Reprod. Advance Access published online on September 2, 2008
Molecular Human Reproduction, doi:10.1093/molehr/gan050
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

The Role of T-lymphoma Invasion and Metastasis Inducing Protein 1 in Early Pregnancy in Mice

Hai-Lan Ma, Tan Zhang, Jun Meng, Zhi-Yuan Qin, Fang Du, Qing-Yue Wang and Sha-Li Wei*

No.1, Xueyuan Road, Yuanjiagang, Yuzhong District, Chongqing 400016, People's Republic of China Department of the Reproductive Physiology, College of Public Health, Chongqing Medical University, Chongqing 400016, People's Republic of China

* Correspondences: Sha-Li WEI, Department of the Reproductive Physiology, College of Public Health, Chongqing Medical University, Chongqing 400016, P. R. China. Email address: zwz007cn{at}yahoo.com.cn

T-lymphoma invasion and metastasis inducing protein 1 (Tiam1) is involved in tumorigenesis processes including cell migration, adhesion and invasion, proteolysis, cytoskeleton reorganization, cell morphological transformation and intracellular signaling. These processes are also critical for embryo implantation, although the role of Tiam1 during embryo implantation remains poorly understood. The aim of this study was to investigate the spatio-temporal expression of Tiam1 in endometria of mice comparing early pregnancy and non-pregnancy. Fluorescent quantitative-PCR and immunohistochemical analysis showed that the expression of Tiam1 mRNA and protein in endometria of pregnant mice was higher than that of non-pregnant mice, and gradually increased from pregnancy day 1 reaching a maximum level on day 5 and then declining on day 6 and 7. Immunohistochemistry showed that Tiam1 protein was present in luminal epithelium from pregnancy day 3 to day 5 and in gland epithelium from day 4 to day 6 and enhanced significantly in stromal cells on day 5, regarded as the "implantation window" period. The number of implantation sites was greatly decreased by the intrauterine injection with anti-Tiam1 polyclonal antibody in the early morning of the pregnancy day 4. These findings suggest that Tiam1 might play an important role in embryo implantation in mice.

Key Words: Embryo/Endometrium/Implantation/Early pregnancy/Tiam1

Submitted on May 9, 2008; resubmitted on August 28, 2008; accepted on August 28, 2008.


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