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Mol. Hum. Reprod. Advance Access published online on December 22, 2008
Molecular Human Reproduction, doi:10.1093/molehr/gan080
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Keratinocyte growth factor and its receptor in human ovaries from fetuses, girls and women

R. Abira,b, B. Fischa,b, X.Y. Zhangc, C. Felza,b, G. Kessler-Iceksone, H. Krissib, S. Nitkeb and A. Aoc,d

aInfertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva, 49100 and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel bHelen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva, 49100 and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel cDepartment of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec H3A 1A1, Canada dDepartment of Human Genetics, McGill University, Montreal, Quebec H3A 1A1, Canada eFelsenstein Medical Research Center, Beilinson Hospital, Petach Tikva 49100 and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

Address for correspondence: Ronit Abir, Ph.D., Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva, 49100, Israel (Fax: 972 3 9376643; e mail: ronita{at}clalit.org.il)

Keratinocyte growth factor (KGF) promotes growth of rat preantral follicles. There is limited information regarding its presence or that of its unique receptor (KGFR) in human ovaries, specifically in preantral follicles. The aim of the study was to investigate the expression of KGF and KGFR in ovarian samples from human fetuses and girls/women. The samples were prepared for immunohistochemical study of the KGF protein and for in situ hybridization to localize mRNA transcripts of KGFR. Total RNA was extracted from frozen ovarian samples, and the expression of KGF mRNA transcripts was investigated by reverse transcriptase polymerase chain reaction. In both fetuses and girls/women the protein for KGF was detected from primordial stages in oocytes, granulosa cells, and stroma cells. Its mRNA transcripts were also detected in all extracts. The mRNA transcripts for KGFR were detected mainly in stroma cells in ovarian samples from both sources; in 10% of the samples, follicular staining was noted also in oocytes and granulosa cells. Further studies adding KGF to the culture medium are needed to elucidate its putative role in human primordial follicle activation.

Key Words: primordial follicles/KGF/KGFR/immunohistochemistry/in situ hybridization/RT-PCR

Submitted on August 11, 2008; resubmitted on November 16, 2008; accepted on December 18, 2008.


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