The cover image shows Fluorescent immunostaining of denudated chorioallantoid membranes. Rat chorioallantoid membranes were cell depleted as described in the Materials and methods and processed for immunostaining. Each panel (A–D) includes, in the same order, an upper right quadrant in which the green signal corresponds to anti-matrix metalloproteinase 9 (MMP-9) and in the upper left quadrant a red signal corresponding to anti-TIMP-2 (D). The red and green channels are shown merged in the lower right quadrants and reflect the co-localization of all the components and MMP-9 (orange/yellow signal). The lower left panel shows phase-contrast microscopy of the double-immunostained sections. Confocal microscopy: magnification ×63. (See N.Meraz-Cruz et al., pp. 633–641).
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