The cover image shows E-cadherin/Connexin 43, E-cadherin/N-cadherin and E-cadherin/β-catenin
immunostaining of human embryonic stem cell (hESC) colonies grown under feeder-free conditions
(VUB03_DM1 at passage 47). Images of the border of hESC colonies switched to feeder-free culture
conditions and fixed after 3 days of culture (A–I). Images scanned by confocal microscopy after
immunostaining with E-cadherin antibody (green) (A, D, G), with Connexin 43 antibody (red) (B),
N-cadherin antibody (red) (E) and β-catenin antibody (red) (H) and merged E-cadherin/Connexin 43
(C), E-cadherin/N-cadherin (F) and E-cadherin/β-catenin (I) immunostaining images. Arrows mark
the periphery of the hESC colonies where mesenchymal-like cells are emerging. Double white arrows
indicate some of the nuclei stained by β-catenin antibody. Original magnification: (A–I) ×200.
(See U.Ullmann
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