The cover image shows Fertilization cones in eggs fertilized by conventional IVF or by ICSI with whole sperm or permeabilized sperm head Eggs were fertilized by conventional IVF (A and B) or by ICSI with whole sperm (B and C) or by ICSI with permeablized sperm (E and F). A, C and E show the localization of F-actin, stained with phalloidin; the fertilization cone is indicated with the asterisk. B, D and F show DNA staining with DAPI. PB2, second polar body (somewhat out of the plane of focus in E and F); M, maternal DNA; S, sperm DNA (See Gao et al., pp. 557-565).
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