The cover image shows expression analyses of FAF1 in the ovary and early preimplantation embryos. (A and B) In the adult ovary, FAF1 immunoreactivity was detected in the cytoplasm of oocytes in the primary (pf), secondary (Sf) and antral follicles (Af). (C-F) FAF1 protein is presence in unfertilized oocytes (C), persists in early zygotes collected 10 h post fertilization (D) and in early cleavage embryos at 2- (E), 4- and 8-cell stage (F). (G) Embryonic Faf1 expression in preimplantation stages. RT-PCR was used to detect Faf1 and Gapdh transcripts in total RNA preparation from wild-type 1- (1), 2- (2), 4- (4), 8-cell stage (8) morula (m), blastocysts (b). Scale bars=200 μm in (A) and (B) (See Adham et al., pp. 207-213).
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